Molecular cloning and characterization of mouse TIE and TEK receptor tyrosine kinase genes and their expression in hematopoietic stem cells

Atsushi Iwama, Isao Hamaguchi, Motohiro Hashiyama, Yuko Murayama, Kunio Yasunaga, Toshio Suda

Research output: Contribution to journalArticle

183 Citations (Scopus)

Abstract

To identify receptor tyrosine kinases (RTKs) critical to early hematopoiesis, we performed polymerase chain reaction-based cloning from yolk sac and highly enriched bone marrow hematopoietic stem cells (HSCs). Characterization of two novel genes of their full-length cDNA sequences revealed that they were mouse homologues of the endothelial cell RTK genes, TIE and TEK. They shared a unique structural property of coexistent immunoglobulin-like domain, epidermal growth factor-like repeats, and fibronectin type III repeats in their extracellular domains. Both genes were expressed in a similar fashion in adult tissues and primitive hematopoietic cells, predominantly in the bone marrow HSCs.

Original languageEnglish
Pages (from-to)301-309
Number of pages9
JournalBiochemical and Biophysical Research Communications
Volume195
Issue number1
DOIs
Publication statusPublished - 1993 Aug 31

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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