Molecular cloning and characterization of the inositol 1,4,5-trisphosphate receptor in Drosophila melanogaster

Shingo Yoshikawa, Teiichi Tanimura, Atsushi Miyawaki, Makoto Nakamura, Michisuke Yuzaki, Teiichi Furuichi, Katsuhiko Mikoshiba

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

We isolated a cDNA encoding an inositol 1,4,5-trisphosphate receptor (InsP3R) of Drosophila melanogaster. The predicted Drosophila InsP3R (2,833 amino acids) has extensive sequence similarity to the mouse InsP3R. The polypeptide encoded by the cDNA was functionally expressed and showed characteristic InsP3-binding activity. The Drosophila InsP3R gene is located at the region 83A5-9 on the third chromosome and expresses throughout development but predominantly in the adult. Localization of the InsP3R mRNA in adult tissues suggests strong expression in the retina and antenna, indicating the involvement of the InsP3R in visual and olfactory transduction. In addition, the InsP3R mRNA is abundant in the legs and thorax, which are enriched with a muscular system. Such localization is apparently consistent with the quantitatively predominant sites for [3H]InsP3 binding in Drosophila and the fleshfly (Boettcherisca peregrina). The present study points to the likely functional importance of the InsP3/Ca2+ signaling system in Drosophila.

Original languageEnglish
Pages (from-to)16613-16619
Number of pages7
JournalJournal of Biological Chemistry
Volume267
Issue number23
Publication statusPublished - 1992 Aug 15
Externally publishedYes

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Inositol 1,4,5-Trisphosphate Receptors
Cloning
Molecular Cloning
Drosophila melanogaster
Drosophila
Complementary DNA
Messenger RNA
Chromosomes
Genes
Sarcophagidae
Tissue
Antennas
Amino Acids
Peptides
Retina
Leg
Thorax

ASJC Scopus subject areas

  • Biochemistry

Cite this

Yoshikawa, S., Tanimura, T., Miyawaki, A., Nakamura, M., Yuzaki, M., Furuichi, T., & Mikoshiba, K. (1992). Molecular cloning and characterization of the inositol 1,4,5-trisphosphate receptor in Drosophila melanogaster. Journal of Biological Chemistry, 267(23), 16613-16619.

Molecular cloning and characterization of the inositol 1,4,5-trisphosphate receptor in Drosophila melanogaster. / Yoshikawa, Shingo; Tanimura, Teiichi; Miyawaki, Atsushi; Nakamura, Makoto; Yuzaki, Michisuke; Furuichi, Teiichi; Mikoshiba, Katsuhiko.

In: Journal of Biological Chemistry, Vol. 267, No. 23, 15.08.1992, p. 16613-16619.

Research output: Contribution to journalArticle

Yoshikawa, S, Tanimura, T, Miyawaki, A, Nakamura, M, Yuzaki, M, Furuichi, T & Mikoshiba, K 1992, 'Molecular cloning and characterization of the inositol 1,4,5-trisphosphate receptor in Drosophila melanogaster', Journal of Biological Chemistry, vol. 267, no. 23, pp. 16613-16619.
Yoshikawa S, Tanimura T, Miyawaki A, Nakamura M, Yuzaki M, Furuichi T et al. Molecular cloning and characterization of the inositol 1,4,5-trisphosphate receptor in Drosophila melanogaster. Journal of Biological Chemistry. 1992 Aug 15;267(23):16613-16619.
Yoshikawa, Shingo ; Tanimura, Teiichi ; Miyawaki, Atsushi ; Nakamura, Makoto ; Yuzaki, Michisuke ; Furuichi, Teiichi ; Mikoshiba, Katsuhiko. / Molecular cloning and characterization of the inositol 1,4,5-trisphosphate receptor in Drosophila melanogaster. In: Journal of Biological Chemistry. 1992 ; Vol. 267, No. 23. pp. 16613-16619.
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