Molecular cloning and chromosomal localization of the ADH7 gene encoding human class IV (σ) ADH

Hirokazu Yokoyama; Enrique Baraona; Charles S. Lieber

doi:10.1006/geno.1996.0040

Molecular cloning and chromosomal localization of the ADH7 gene encoding human class IV (σ) ADH

Hirokazu Yokoyama, Enrique Baraona, Charles S. Lieber

Research output: Contribution to journal › Article › peer-review

Abstract

The ADH7 gene encoding human Class IV (σ) alcohol dehydrogenase (ADH) was cloned from a Caucasian genomic DNA library and characterized. It has nine exons and eight introns that span about 22 kb, and its intron insertion is identical to that of the other ADH genes (ADH1 to ADH5). The nucleotide sequences of the exons encoding 374 amino acids are identical to the previously reported cDNA sequence of σ ADH. Fluorescence in situ hybridization analysis showed that ADH7 is located on human chromosome 4q23- q24, close to the ADH cluster locus (4q21-q25). These data are consistent with the view that Class IV ADH is a member of the ADH family and is phylogenetically close to the other ADHs.

Original language	English
Pages (from-to)	243-245
Number of pages	3
Journal	Genomics
Volume	31
Issue number	2
DOIs	https://doi.org/10.1006/geno.1996.0040
Publication status	Published - 1996 Jan 15
Externally published	Yes

ASJC Scopus subject areas

Genetics

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title = "Molecular cloning and chromosomal localization of the ADH7 gene encoding human class IV (σ) ADH",

abstract = "The ADH7 gene encoding human Class IV (σ) alcohol dehydrogenase (ADH) was cloned from a Caucasian genomic DNA library and characterized. It has nine exons and eight introns that span about 22 kb, and its intron insertion is identical to that of the other ADH genes (ADH1 to ADH5). The nucleotide sequences of the exons encoding 374 amino acids are identical to the previously reported cDNA sequence of σ ADH. Fluorescence in situ hybridization analysis showed that ADH7 is located on human chromosome 4q23- q24, close to the ADH cluster locus (4q21-q25). These data are consistent with the view that Class IV ADH is a member of the ADH family and is phylogenetically close to the other ADHs.",

author = "Hirokazu Yokoyama and Enrique Baraona and Lieber, {Charles S.}",

note = "Funding Information: This study was supported by NIH Grants AA 03508, AA 05934, and the Department of Veterans Affairs.",

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AU - Yokoyama, Hirokazu

AU - Baraona, Enrique

AU - Lieber, Charles S.

N1 - Funding Information: This study was supported by NIH Grants AA 03508, AA 05934, and the Department of Veterans Affairs.

PY - 1996/1/15

Y1 - 1996/1/15

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AB - The ADH7 gene encoding human Class IV (σ) alcohol dehydrogenase (ADH) was cloned from a Caucasian genomic DNA library and characterized. It has nine exons and eight introns that span about 22 kb, and its intron insertion is identical to that of the other ADH genes (ADH1 to ADH5). The nucleotide sequences of the exons encoding 374 amino acids are identical to the previously reported cDNA sequence of σ ADH. Fluorescence in situ hybridization analysis showed that ADH7 is located on human chromosome 4q23- q24, close to the ADH cluster locus (4q21-q25). These data are consistent with the view that Class IV ADH is a member of the ADH family and is phylogenetically close to the other ADHs.

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Molecular cloning and chromosomal localization of the ADH7 gene encoding human class IV (σ) ADH

Abstract

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