Molecular cloning and chromosomal mapping of a cDNA encoding human 80K-L protein

Major substrate for protein kinase C

Kosuke Sakai, Masamichi Hirai, Jun Kudo, Shinsei Minoshima, Nobuyoshi Shimizu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We have isolated and sequenced complementary DNA (cDNA) for the human 80K-L protein, a major substrate for protein kinase C and the human homologue of an 80- to 87-kDa bovine protein named MARCKS (myristoylated alanine-rich C kinase substrate). The human 80K-L cDNA encodes a protein of 332 amino acids with a calculated molecular weight of 31,534. Homology comparisons of the nucleotide sequences of the cDNAs indicated that their 3′-untranslated regions are more homologous than the coding regions. Spot blot hybridization using flow-sorted human chromosomes indicated that the gene encoding the 80K-L protein, designated MACS, maps to the q15 → qter region of human chromosome 6, and it also suggested that a genomic region with a sequence homologous to the 3′-untranslated region of the 80K-L mRNA exists on chromosome 21.

Original languageEnglish
Pages (from-to)175-178
Number of pages4
JournalGenomics
Volume14
Issue number1
DOIs
Publication statusPublished - 1992

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Molecular Cloning
Protein Kinase C
Complementary DNA
Human Chromosomes
3' Untranslated Regions
Chromosomes, Human, Pair 21
Chromosomes, Human, Pair 6
Proteins
Sequence Homology
Molecular Weight
Amino Acids
Messenger RNA
Genes
myristoylated alanine-rich C kinase substrate

ASJC Scopus subject areas

  • Genetics

Cite this

Molecular cloning and chromosomal mapping of a cDNA encoding human 80K-L protein : Major substrate for protein kinase C. / Sakai, Kosuke; Hirai, Masamichi; Kudo, Jun; Minoshima, Shinsei; Shimizu, Nobuyoshi.

In: Genomics, Vol. 14, No. 1, 1992, p. 175-178.

Research output: Contribution to journalArticle

Sakai, Kosuke ; Hirai, Masamichi ; Kudo, Jun ; Minoshima, Shinsei ; Shimizu, Nobuyoshi. / Molecular cloning and chromosomal mapping of a cDNA encoding human 80K-L protein : Major substrate for protein kinase C. In: Genomics. 1992 ; Vol. 14, No. 1. pp. 175-178.
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