Molecular cloning and chromosomal mapping of a cDNA encoding human 80K-L protein: Major substrate for protein kinase C

Kosuke Sakai, Masamichi Hirai, Jun Kudoh, Shinsei Minoshima, Nobuyoshi Shimizu

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We have isolated and sequenced complementary DNA (cDNA) for the human 80K-L protein, a major substrate for protein kinase C and the human homologue of an 80- to 87-kDa bovine protein named MARCKS (myristoylated alanine-rich C kinase substrate). The human 80K-L cDNA encodes a protein of 332 amino acids with a calculated molecular weight of 31,534. Homology comparisons of the nucleotide sequences of the cDNAs indicated that their 3′-untranslated regions are more homologous than the coding regions. Spot blot hybridization using flow-sorted human chromosomes indicated that the gene encoding the 80K-L protein, designated MACS, maps to the q15 → qter region of human chromosome 6, and it also suggested that a genomic region with a sequence homologous to the 3′-untranslated region of the 80K-L mRNA exists on chromosome 21.

Original languageEnglish
Pages (from-to)175-178
Number of pages4
Issue number1
Publication statusPublished - 1992 Sep


ASJC Scopus subject areas

  • Genetics

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