Molecular cloning and chromosomal mapping of mouse intronless myc gene acting as a potent apoptosis inducer

Akinori Sugiyama, Kohji Noguchi, Chifumi Kitanaka, Naoko Katou, Fumio Tashiro, Takeo Ono, Michihiro C. Yoshida, Yoshiyuki Kuchino

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Our previous findings suggest that the activation of the rat intronless myc gene provides a selective advantage in tumor suppression through apoptosis induction. In the present study, to examine whether intronless myc gene acting as an apoptosis inducer is evolutionarily conserved in mammalian cells, we isolated the mouse intronless myc gene and characterized it. A sequence analysis demonstrated that mouse intronless myc gene, ms-myc, has a linearly opened translatable frame consisting of 1293 bp with 90% homology with that of rat s-myc. The chromosomal locus of ms-myc was identified on chromosome 19B by a fluorescent in situ hybridization (FISH) analysis. Gene transfection experiments showed that the transient overexpression of ms-Myc with transactivation activity effectively induces cell death in a wild-type p53-independent manner. In addition, cells stably expressing transfected ms- myc became more susceptible to apoptosis induced by genotoxic stress such as UV-irradiation and hydrogen peroxide compared with untransfected control cells. These observations suggest that the rodents commonly contain an s- myc-type of intronless myc gene with apoptosis-inducing activity.

Original languageEnglish
Pages (from-to)273-283
Number of pages11
JournalGene
Volume226
Issue number2
DOIs
Publication statusPublished - 1999 Jan 21
Externally publishedYes

Fingerprint

myc Genes
Molecular Cloning
Apoptosis
Fluorescence In Situ Hybridization
Hydrogen Peroxide
Transcriptional Activation
DNA Damage
Transfection
Sequence Analysis
Rodentia
Cell Death
Chromosomes
Genes
Neoplasms

Keywords

  • FISH analysis
  • Molecular evolution
  • Myc Family gene
  • Programmed cell death
  • Transactivation factor

ASJC Scopus subject areas

  • Genetics

Cite this

Sugiyama, A., Noguchi, K., Kitanaka, C., Katou, N., Tashiro, F., Ono, T., ... Kuchino, Y. (1999). Molecular cloning and chromosomal mapping of mouse intronless myc gene acting as a potent apoptosis inducer. Gene, 226(2), 273-283. https://doi.org/10.1016/S0378-1119(98)00547-2

Molecular cloning and chromosomal mapping of mouse intronless myc gene acting as a potent apoptosis inducer. / Sugiyama, Akinori; Noguchi, Kohji; Kitanaka, Chifumi; Katou, Naoko; Tashiro, Fumio; Ono, Takeo; Yoshida, Michihiro C.; Kuchino, Yoshiyuki.

In: Gene, Vol. 226, No. 2, 21.01.1999, p. 273-283.

Research output: Contribution to journalArticle

Sugiyama, A, Noguchi, K, Kitanaka, C, Katou, N, Tashiro, F, Ono, T, Yoshida, MC & Kuchino, Y 1999, 'Molecular cloning and chromosomal mapping of mouse intronless myc gene acting as a potent apoptosis inducer', Gene, vol. 226, no. 2, pp. 273-283. https://doi.org/10.1016/S0378-1119(98)00547-2
Sugiyama, Akinori ; Noguchi, Kohji ; Kitanaka, Chifumi ; Katou, Naoko ; Tashiro, Fumio ; Ono, Takeo ; Yoshida, Michihiro C. ; Kuchino, Yoshiyuki. / Molecular cloning and chromosomal mapping of mouse intronless myc gene acting as a potent apoptosis inducer. In: Gene. 1999 ; Vol. 226, No. 2. pp. 273-283.
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