Molecular cloning and expression of a cDNA encoding an olfactory-specific mouse phenol sulphotransferase

Hiroomi Tamura, Yuki Harada, Atsushi Miyawaki, Katsuhiko Mikoshiba, Michio Matsui

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Previously we demonstrated the presence of phenol sulphotransferase (P-ST) in mouse nasal cytosols and identified its zonal location in mouse nasal cavity by staining with an antiserum raised against a rat liver P-ST isoenzyme.,PSTG. In the present study a cDNA was isolated from a mouse olfactory cDNA library by immunological screening with the antiserum. The isolated cDNA consisted of 1347 bp with a 912 bp open reading frame encoding a 304-residue polypeptide. Both the nucleotide and deduced amino acid sequences of the cDNA were 94% identical with those of a rat liver P-ST isoenzyme, ST1C1. The expressed enzyme in Escherichia coli displayed high P-ST activity towards phenolic odorants such as eugenol and guaiacol, and it showed a high N-hydroxy-2-acetylaminofluorene sulphation activity in comparison with the rat ST1C1 enzyme. These results indicate that the olfactory P-ST encoded by the cDNA is a mouse orthologue of rat ST1C1; however, expression of the olfactory P-ST mRNA is specific for nasal tissues as revealed by reverse transcriptase-mediated PCR (RT-PCR).

Original languageEnglish
Pages (from-to)953-958
Number of pages6
JournalBiochemical Journal
Volume331
Issue number3
Publication statusPublished - 1998 May 1

Fingerprint

Arylsulfotransferase
Cloning
Molecular Cloning
Complementary DNA
Rats
Nose
Liver
Isoenzymes
Immune Sera
Guaiacol
2-Acetylaminofluorene
Eugenol
Nasal Cavity
RNA-Directed DNA Polymerase
Enzymes
Reverse Transcriptase Polymerase Chain Reaction
Gene Library
Cytosol
Escherichia coli
Open Reading Frames

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molecular cloning and expression of a cDNA encoding an olfactory-specific mouse phenol sulphotransferase. / Tamura, Hiroomi; Harada, Yuki; Miyawaki, Atsushi; Mikoshiba, Katsuhiko; Matsui, Michio.

In: Biochemical Journal, Vol. 331, No. 3, 01.05.1998, p. 953-958.

Research output: Contribution to journalArticle

Tamura, H, Harada, Y, Miyawaki, A, Mikoshiba, K & Matsui, M 1998, 'Molecular cloning and expression of a cDNA encoding an olfactory-specific mouse phenol sulphotransferase', Biochemical Journal, vol. 331, no. 3, pp. 953-958.
Tamura, Hiroomi ; Harada, Yuki ; Miyawaki, Atsushi ; Mikoshiba, Katsuhiko ; Matsui, Michio. / Molecular cloning and expression of a cDNA encoding an olfactory-specific mouse phenol sulphotransferase. In: Biochemical Journal. 1998 ; Vol. 331, No. 3. pp. 953-958.
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