Molecular cloning and functional characterization of a novel member of the C-C chemokine family

T. Hara, K. B. Bacon, L. C. Cho, Akihiko Yoshimura, Y. Morikawa, N. G. Copeland, D. J. Gilbert, N. A. Jenkins, T. J. Schall, A. Miyajima

Research output: Contribution to journalArticle

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Abstract

Chemokines play an important role in immune and inflammatory responses by inducing migration and adhesion of leukocytes. We have isolated a novel chemokine cDNA, designated CCF18, from a cDNA library of an IL-3-dependent murine pro-B cell line, Ba/F3. The cDNA encodes a protein structurally related to the C-C chemokine members. Among this family, C10 shows the highest homology to CCF18, and MIP-1α also has a significant homology but to a lesser extent. CCF18 produced from COS cells induced chemotaxis and Ca2+ flux in CD4+ T cell clones. Moreover, prior administration of MIP-1α desensitized the cells to CCF18. The CCF18 gene (Scya10) was mapped to a middle region of murine chromosome 11, where other genes for several C-C chemokine members are localized. These results clearly indicate that CCF18 is a new member of the C-C chemokine family. Since a high level of CCF18 mRNA is constitutively expressed in macrophage and myeloid cell lines, CCF18 may play a role in inflammatory processes.

Original languageEnglish
Pages (from-to)5352-5358
Number of pages7
JournalJournal of Immunology
Volume155
Issue number11
Publication statusPublished - 1995
Externally publishedYes

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CC Chemokines
Molecular Cloning
Chemokines
Complementary DNA
Cell Line
Chromosomes, Human, Pair 11
B-Lymphoid Precursor Cells
Interleukin-3
COS Cells
Chemotaxis
Myeloid Cells
Gene Library
Genes
Leukocytes
Clone Cells
Macrophages
T-Lymphocytes
Messenger RNA
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Hara, T., Bacon, K. B., Cho, L. C., Yoshimura, A., Morikawa, Y., Copeland, N. G., ... Miyajima, A. (1995). Molecular cloning and functional characterization of a novel member of the C-C chemokine family. Journal of Immunology, 155(11), 5352-5358.

Molecular cloning and functional characterization of a novel member of the C-C chemokine family. / Hara, T.; Bacon, K. B.; Cho, L. C.; Yoshimura, Akihiko; Morikawa, Y.; Copeland, N. G.; Gilbert, D. J.; Jenkins, N. A.; Schall, T. J.; Miyajima, A.

In: Journal of Immunology, Vol. 155, No. 11, 1995, p. 5352-5358.

Research output: Contribution to journalArticle

Hara, T, Bacon, KB, Cho, LC, Yoshimura, A, Morikawa, Y, Copeland, NG, Gilbert, DJ, Jenkins, NA, Schall, TJ & Miyajima, A 1995, 'Molecular cloning and functional characterization of a novel member of the C-C chemokine family', Journal of Immunology, vol. 155, no. 11, pp. 5352-5358.
Hara, T. ; Bacon, K. B. ; Cho, L. C. ; Yoshimura, Akihiko ; Morikawa, Y. ; Copeland, N. G. ; Gilbert, D. J. ; Jenkins, N. A. ; Schall, T. J. ; Miyajima, A. / Molecular cloning and functional characterization of a novel member of the C-C chemokine family. In: Journal of Immunology. 1995 ; Vol. 155, No. 11. pp. 5352-5358.
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AU - Bacon, K. B.

AU - Cho, L. C.

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AU - Morikawa, Y.

AU - Copeland, N. G.

AU - Gilbert, D. J.

AU - Jenkins, N. A.

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