Molecular cloning and nucleotide sequence of a cDNA encoding Euglena gracilis Cytochrome C1

Kuniaki Mukai, Sadao Wakabayashi, Hiroshi Matsubara

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The amino acid sequence of the mature protein of Euglena gracilis cytochrome c1 was determined by sequencing of its cDNA. A cDNA expression library was constructed from Euglena poly(A)+ RNA in phage λgt11 and screened with an antiserum raised against cytochrome c1 polypeptide isolated from purified E. gracilis complex III. An isolated cDNA clone consisted of 872 base pairs and encoded the mature protein with 243 amino acids. The deduced amino acid sequence contained the unusual heme binding sequence -Phe-Ala-Pro-Cys-His- (Mukai, K. et al (1989) Eur. J. Biochem. 178, 649-656) instead of the typical sequence, -Cys-X-Y-Cys-His-, commonly found in C-type cytochromes. Comparison of the sequence with those of several other cytochromes c1 revealed that Euglena cytochrome c1 conserved the residues probably ligating heme-iron, those supposed to interact with cytochrome c and regions anchoring the mitochondrial inner membrane.

Original languageEnglish
Pages (from-to)479-482
Number of pages4
JournalJournal of Biochemistry
Volume106
Issue number3
Publication statusPublished - 1989 Sep
Externally publishedYes

Fingerprint

Euglena Gracilis
Euglena gracilis
Cytochromes c1
Cloning
Molecular Cloning
Nucleotides
CDNA
Encoding
Complementary DNA
Euglena
Amino Acid Sequence
Proteins
Heme
Amino Acids
Protein
RNA Phages
Clone
Amino acids
Iron
Sequencing

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Molecular cloning and nucleotide sequence of a cDNA encoding Euglena gracilis Cytochrome C1. / Mukai, Kuniaki; Wakabayashi, Sadao; Matsubara, Hiroshi.

In: Journal of Biochemistry, Vol. 106, No. 3, 09.1989, p. 479-482.

Research output: Contribution to journalArticle

Mukai, Kuniaki ; Wakabayashi, Sadao ; Matsubara, Hiroshi. / Molecular cloning and nucleotide sequence of a cDNA encoding Euglena gracilis Cytochrome C1. In: Journal of Biochemistry. 1989 ; Vol. 106, No. 3. pp. 479-482.
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