Molecular cloning and sequence analysis of the DnaA gene of staphylococcus aureus

Hideki Katayama, Tohru Mizushima, Takeyoshi Miki, Kazuhisa Sekimizu

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5 Citations (Scopus)

Abstract

The dnaA gene of Staphylococcus aureus has now been cloned and sequenced. The deduced amino acid sequence of Staphylococcus aureus DnaA protein is 62% and 39% identical to those of the DnaA protein from Bacillus subtilis and Escherichia coli, respectively. Expression of the dnaA gene of Staphylococcus aureus in Escherichia coli caused lethality in an oriC-dependent manner.

Original languageEnglish
Pages (from-to)820-822
Number of pages3
JournalBiological and Pharmaceutical Bulletin
Volume20
Issue number7
Publication statusPublished - 1997
Externally publishedYes

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Keywords

  • DnaA protein
  • Molecular cloning
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Katayama, H., Mizushima, T., Miki, T., & Sekimizu, K. (1997). Molecular cloning and sequence analysis of the DnaA gene of staphylococcus aureus. Biological and Pharmaceutical Bulletin, 20(7), 820-822.