Abstract
Limonene is one of the most simple cyclic monoterpenes, and two enantiomers, d- and l-limonene occur due to the chiral carbon at 4-position. Cyclization of GPP into limonene is catalyzed by the limonene synthase, and some l-limonene synthase cDNAs have already been cloned from several species of plants, mainly from Labiatae family. However, the d-limonene synthase gene has not yet been obtained, therefore, no information is available on the molecular mechanism of stereochemical regulation in limonene formation. To resolve this, we cloned the d-limonene synthase gene (dLMS) from Schizonepeta tenuifolia (Labiatae) by a reverse genetic approach, and we found that both d- and l-limonene synthase share similar features such as a transit peptide, an arginine rich domain, and a metal cation binding site in their structures. Here, we report on the cloning of dLMS, and the putative stereochemical regulation mechanism is discussed based on the comparison of the deduced amino acid sequence of dLMS with those of known l-limonene synthase.
| Original language | English |
|---|---|
| Pages (from-to) | 373-377 |
| Number of pages | 5 |
| Journal | Biological and Pharmaceutical Bulletin |
| Volume | 24 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - 2001 |
| Externally published | Yes |
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Keywords
- CDNA cloning
- D-limonene synthase
- Monoterpene biosynthesis
- Schizonepeta tenuifolia (Labiatae)
ASJC Scopus subject areas
- Molecular Medicine
- Pharmacology, Toxicology and Pharmaceutics(all)
Cite this
Molecular cloning, functional expression and characterization of d-limonene synthase from Schizonepeta tenuifolia. / Maruyama, T.; Ito, M.; Kiuchi, Fumiyuki; Honda, G.
In: Biological and Pharmaceutical Bulletin, Vol. 24, No. 4, 2001, p. 373-377.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular cloning, functional expression and characterization of d-limonene synthase from Schizonepeta tenuifolia
AU - Maruyama, T.
AU - Ito, M.
AU - Kiuchi, Fumiyuki
AU - Honda, G.
PY - 2001
Y1 - 2001
N2 - Limonene is one of the most simple cyclic monoterpenes, and two enantiomers, d- and l-limonene occur due to the chiral carbon at 4-position. Cyclization of GPP into limonene is catalyzed by the limonene synthase, and some l-limonene synthase cDNAs have already been cloned from several species of plants, mainly from Labiatae family. However, the d-limonene synthase gene has not yet been obtained, therefore, no information is available on the molecular mechanism of stereochemical regulation in limonene formation. To resolve this, we cloned the d-limonene synthase gene (dLMS) from Schizonepeta tenuifolia (Labiatae) by a reverse genetic approach, and we found that both d- and l-limonene synthase share similar features such as a transit peptide, an arginine rich domain, and a metal cation binding site in their structures. Here, we report on the cloning of dLMS, and the putative stereochemical regulation mechanism is discussed based on the comparison of the deduced amino acid sequence of dLMS with those of known l-limonene synthase.
AB - Limonene is one of the most simple cyclic monoterpenes, and two enantiomers, d- and l-limonene occur due to the chiral carbon at 4-position. Cyclization of GPP into limonene is catalyzed by the limonene synthase, and some l-limonene synthase cDNAs have already been cloned from several species of plants, mainly from Labiatae family. However, the d-limonene synthase gene has not yet been obtained, therefore, no information is available on the molecular mechanism of stereochemical regulation in limonene formation. To resolve this, we cloned the d-limonene synthase gene (dLMS) from Schizonepeta tenuifolia (Labiatae) by a reverse genetic approach, and we found that both d- and l-limonene synthase share similar features such as a transit peptide, an arginine rich domain, and a metal cation binding site in their structures. Here, we report on the cloning of dLMS, and the putative stereochemical regulation mechanism is discussed based on the comparison of the deduced amino acid sequence of dLMS with those of known l-limonene synthase.
KW - CDNA cloning
KW - D-limonene synthase
KW - Monoterpene biosynthesis
KW - Schizonepeta tenuifolia (Labiatae)
UR - http://www.scopus.com/inward/record.url?scp=0035028219&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035028219&partnerID=8YFLogxK
U2 - 10.1248/bpb.24.373
DO - 10.1248/bpb.24.373
M3 - Article
C2 - 11305598
AN - SCOPUS:0035028219
VL - 24
SP - 373
EP - 377
JO - Biological and Pharmaceutical Bulletin
JF - Biological and Pharmaceutical Bulletin
SN - 0918-6158
IS - 4
ER -