Molecular cloning, functional expression and characterization of d-limonene synthase from Schizonepeta tenuifolia

T. Maruyama, M. Ito, Fumiyuki Kiuchi, G. Honda

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Limonene is one of the most simple cyclic monoterpenes, and two enantiomers, d- and l-limonene occur due to the chiral carbon at 4-position. Cyclization of GPP into limonene is catalyzed by the limonene synthase, and some l-limonene synthase cDNAs have already been cloned from several species of plants, mainly from Labiatae family. However, the d-limonene synthase gene has not yet been obtained, therefore, no information is available on the molecular mechanism of stereochemical regulation in limonene formation. To resolve this, we cloned the d-limonene synthase gene (dLMS) from Schizonepeta tenuifolia (Labiatae) by a reverse genetic approach, and we found that both d- and l-limonene synthase share similar features such as a transit peptide, an arginine rich domain, and a metal cation binding site in their structures. Here, we report on the cloning of dLMS, and the putative stereochemical regulation mechanism is discussed based on the comparison of the deduced amino acid sequence of dLMS with those of known l-limonene synthase.

Original languageEnglish
Pages (from-to)373-377
Number of pages5
JournalBiological and Pharmaceutical Bulletin
Volume24
Issue number4
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Lamiaceae
Molecular Cloning
Genes
limonene
pinene cyclase I
Reverse Genetics
Monoterpenes
Cyclization
Arginine
Cations
Organism Cloning
Amino Acid Sequence
Carbon
Complementary DNA
Metals
Binding Sites
Peptides

Keywords

  • CDNA cloning
  • D-limonene synthase
  • Monoterpene biosynthesis
  • Schizonepeta tenuifolia (Labiatae)

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Molecular cloning, functional expression and characterization of d-limonene synthase from Schizonepeta tenuifolia. / Maruyama, T.; Ito, M.; Kiuchi, Fumiyuki; Honda, G.

In: Biological and Pharmaceutical Bulletin, Vol. 24, No. 4, 2001, p. 373-377.

Research output: Contribution to journalArticle

@article{f57d092fa79148b3816093131bdf8784,
title = "Molecular cloning, functional expression and characterization of d-limonene synthase from Schizonepeta tenuifolia",
abstract = "Limonene is one of the most simple cyclic monoterpenes, and two enantiomers, d- and l-limonene occur due to the chiral carbon at 4-position. Cyclization of GPP into limonene is catalyzed by the limonene synthase, and some l-limonene synthase cDNAs have already been cloned from several species of plants, mainly from Labiatae family. However, the d-limonene synthase gene has not yet been obtained, therefore, no information is available on the molecular mechanism of stereochemical regulation in limonene formation. To resolve this, we cloned the d-limonene synthase gene (dLMS) from Schizonepeta tenuifolia (Labiatae) by a reverse genetic approach, and we found that both d- and l-limonene synthase share similar features such as a transit peptide, an arginine rich domain, and a metal cation binding site in their structures. Here, we report on the cloning of dLMS, and the putative stereochemical regulation mechanism is discussed based on the comparison of the deduced amino acid sequence of dLMS with those of known l-limonene synthase.",
keywords = "CDNA cloning, D-limonene synthase, Monoterpene biosynthesis, Schizonepeta tenuifolia (Labiatae)",
author = "T. Maruyama and M. Ito and Fumiyuki Kiuchi and G. Honda",
year = "2001",
doi = "10.1248/bpb.24.373",
language = "English",
volume = "24",
pages = "373--377",
journal = "Biological and Pharmaceutical Bulletin",
issn = "0918-6158",
publisher = "Pharmaceutical Society of Japan",
number = "4",

}

TY - JOUR

T1 - Molecular cloning, functional expression and characterization of d-limonene synthase from Schizonepeta tenuifolia

AU - Maruyama, T.

AU - Ito, M.

AU - Kiuchi, Fumiyuki

AU - Honda, G.

PY - 2001

Y1 - 2001

N2 - Limonene is one of the most simple cyclic monoterpenes, and two enantiomers, d- and l-limonene occur due to the chiral carbon at 4-position. Cyclization of GPP into limonene is catalyzed by the limonene synthase, and some l-limonene synthase cDNAs have already been cloned from several species of plants, mainly from Labiatae family. However, the d-limonene synthase gene has not yet been obtained, therefore, no information is available on the molecular mechanism of stereochemical regulation in limonene formation. To resolve this, we cloned the d-limonene synthase gene (dLMS) from Schizonepeta tenuifolia (Labiatae) by a reverse genetic approach, and we found that both d- and l-limonene synthase share similar features such as a transit peptide, an arginine rich domain, and a metal cation binding site in their structures. Here, we report on the cloning of dLMS, and the putative stereochemical regulation mechanism is discussed based on the comparison of the deduced amino acid sequence of dLMS with those of known l-limonene synthase.

AB - Limonene is one of the most simple cyclic monoterpenes, and two enantiomers, d- and l-limonene occur due to the chiral carbon at 4-position. Cyclization of GPP into limonene is catalyzed by the limonene synthase, and some l-limonene synthase cDNAs have already been cloned from several species of plants, mainly from Labiatae family. However, the d-limonene synthase gene has not yet been obtained, therefore, no information is available on the molecular mechanism of stereochemical regulation in limonene formation. To resolve this, we cloned the d-limonene synthase gene (dLMS) from Schizonepeta tenuifolia (Labiatae) by a reverse genetic approach, and we found that both d- and l-limonene synthase share similar features such as a transit peptide, an arginine rich domain, and a metal cation binding site in their structures. Here, we report on the cloning of dLMS, and the putative stereochemical regulation mechanism is discussed based on the comparison of the deduced amino acid sequence of dLMS with those of known l-limonene synthase.

KW - CDNA cloning

KW - D-limonene synthase

KW - Monoterpene biosynthesis

KW - Schizonepeta tenuifolia (Labiatae)

UR - http://www.scopus.com/inward/record.url?scp=0035028219&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035028219&partnerID=8YFLogxK

U2 - 10.1248/bpb.24.373

DO - 10.1248/bpb.24.373

M3 - Article

C2 - 11305598

AN - SCOPUS:0035028219

VL - 24

SP - 373

EP - 377

JO - Biological and Pharmaceutical Bulletin

JF - Biological and Pharmaceutical Bulletin

SN - 0918-6158

IS - 4

ER -