Molecular cloning of a calmodulin-dependent phosphatase from murine testis: Identification of a developmentally expressed nonneural isoenzyme

Taro Muramatsu, Polavarapu Rathna Giri, Susumu Higuchi, Randall L. Kincaid

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

A unique isoform of the catalytic subunit of calmodulin-dependent protein phosphatase (CaM-PrP) was cloned from a murine testis library. The cDNA sequence of 1964 base pairs contained an open reading frame encoding a protein of 513 amino acids (Mr ≈ 58,706), the predicted isoelectric point of which (pi 7.1) was much more basic than those of brain isoforms (pI 5.6-5.8). The deduced amino acid sequence was 77-81% identical to two other murine CaM-PrP genes and displayed a distinct Southern blot hybridization pattern, indicating that it was derived from a separate gene (type 3). High amounts of a 2800-nucleotide mRNA transcript were observed in testis, whereas mRNA species were not detectable in brain; thus, it seems likely that this CaM-PrP represents a nonneural isoenzyme. Measurements of CaM-PrP mRNA during testicular development showed a dramatic increase in expression during weeks 4-6, correlating with the later stages of spermatogenesis. These data suggest that this phosphatase isoform may be involved in germ-cell function and are consistent with the report of a flagellum-associated form of CaM-PrP that may regulate sperm motility [Tash, J. S., Krinks, M., Patel, J., Means, R. L., Klee, C. B. & Means, A. R. (1988) J. Cell Biol. 106, 1625-1633]. (.

Original languageEnglish
Pages (from-to)529-533
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number2
Publication statusPublished - 1992
Externally publishedYes

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Phosphoprotein Phosphatases
Molecular Cloning
Calmodulin
Phosphoric Monoester Hydrolases
Isoenzymes
Testis
Protein Isoforms
Messenger RNA
Flagella
Sperm Motility
Isoelectric Point
Brain
Spermatogenesis
Southern Blotting
Germ Cells
Base Pairing
Open Reading Frames
Genes
Amino Acid Sequence
Catalytic Domain

Keywords

  • Calcineurin
  • Gene diversity
  • Phosphoproteins
  • Spermatogenesis

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Molecular cloning of a calmodulin-dependent phosphatase from murine testis : Identification of a developmentally expressed nonneural isoenzyme. / Muramatsu, Taro; Giri, Polavarapu Rathna; Higuchi, Susumu; Kincaid, Randall L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, No. 2, 1992, p. 529-533.

Research output: Contribution to journalArticle

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