Molecular cloning of a novel putative Ca2+ channel protein (TRPC7) highly expressed in brain

Kentaro Nagamine, Jun Kudo, Shinsei Minoshima, Kazuhiko Kawasaki, Shuichi Asakawa, Fumiaki Ito, Nobuyoshi Shimizu

Research output: Contribution to journalArticle

184 Citations (Scopus)

Abstract

We have isolated cDNA clones for a novel human protein, TRPC7 (transient receptor potential-related channels), which consists of 1503 amino acid residues from the fetal brain and caudate nucleus cDNA libraries. Northern blot analysis indicated that the TRPC7 gene is highly expressed as a 6.5-kb transcript in brain. The TRPC7 protein has significant homology with Caenorhabditis elegans hypothetical proteins T01H8.5, C05C12.3, and F54D1.5 and with Drosophila and human transient receptor potential (trp) proteins. The TRPC7 protein has seven putative transmembrane domains that probably constitute a Ca2+ channel as in the above-mentioned proteins. Genomic sequencing revealed that the TRPC7 gene consists of 32 exons spanning approximately 90 kb. The TRPC7 gene was mapped between D21S400 and D21S171 on human chromosome 21q22.3, 14 kb distal to a NotI site in D21S400. This novel TRPC7 gene could be a candidate gene for genetic disorders such as bipolar affective disorder, nonsyndromic hereditary deafness, Knobloch syndrome, and holoprosencephaly, which were mapped to this region.

Original languageEnglish
Pages (from-to)124-131
Number of pages8
JournalGenomics
Volume54
Issue number1
DOIs
Publication statusPublished - 1998 Nov 15

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Molecular Cloning
Brain
Genes
Proteins
Caenorhabditis elegans Proteins
Holoprosencephaly
Transient Receptor Potential Channels
Chromosomes, Human, Pair 14
Inborn Genetic Diseases
Caudate Nucleus
Human Chromosomes
Mood Disorders
Gene Library
Bipolar Disorder
Northern Blotting
Exons
Complementary DNA
Clone Cells
Amino Acids

ASJC Scopus subject areas

  • Genetics

Cite this

Nagamine, K., Kudo, J., Minoshima, S., Kawasaki, K., Asakawa, S., Ito, F., & Shimizu, N. (1998). Molecular cloning of a novel putative Ca2+ channel protein (TRPC7) highly expressed in brain. Genomics, 54(1), 124-131. https://doi.org/10.1006/geno.1998.5551

Molecular cloning of a novel putative Ca2+ channel protein (TRPC7) highly expressed in brain. / Nagamine, Kentaro; Kudo, Jun; Minoshima, Shinsei; Kawasaki, Kazuhiko; Asakawa, Shuichi; Ito, Fumiaki; Shimizu, Nobuyoshi.

In: Genomics, Vol. 54, No. 1, 15.11.1998, p. 124-131.

Research output: Contribution to journalArticle

Nagamine, K, Kudo, J, Minoshima, S, Kawasaki, K, Asakawa, S, Ito, F & Shimizu, N 1998, 'Molecular cloning of a novel putative Ca2+ channel protein (TRPC7) highly expressed in brain', Genomics, vol. 54, no. 1, pp. 124-131. https://doi.org/10.1006/geno.1998.5551
Nagamine K, Kudo J, Minoshima S, Kawasaki K, Asakawa S, Ito F et al. Molecular cloning of a novel putative Ca2+ channel protein (TRPC7) highly expressed in brain. Genomics. 1998 Nov 15;54(1):124-131. https://doi.org/10.1006/geno.1998.5551
Nagamine, Kentaro ; Kudo, Jun ; Minoshima, Shinsei ; Kawasaki, Kazuhiko ; Asakawa, Shuichi ; Ito, Fumiaki ; Shimizu, Nobuyoshi. / Molecular cloning of a novel putative Ca2+ channel protein (TRPC7) highly expressed in brain. In: Genomics. 1998 ; Vol. 54, No. 1. pp. 124-131.
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