TY - JOUR
T1 - Molecular Cloning of an Amylase Gene of Bacillus circulans
AU - Nishizawa, Masafumi
AU - Ozawa, Fumiko
AU - Hishinuma, Fumio
PY - 1987/6
Y1 - 1987/6
N2 - An amylase gene of Bacillus circulans was cloned in B. subtilis and its nucleotide sequence was determined. The putative proamylase consists of 528 amino acids, which correspond to a molecular weight of 58,776. Homologous regions with other amylases of Bacillus species were found. A σ55-type promoter is located at about 250 bp upstream from the starting codon. This promoter was also functional in Escherichia coli, and able to express β-galactosidase activity.
AB - An amylase gene of Bacillus circulans was cloned in B. subtilis and its nucleotide sequence was determined. The putative proamylase consists of 528 amino acids, which correspond to a molecular weight of 58,776. Homologous regions with other amylases of Bacillus species were found. A σ55-type promoter is located at about 250 bp upstream from the starting codon. This promoter was also functional in Escherichia coli, and able to express β-galactosidase activity.
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U2 - 10.1089/dna.1987.6.255
DO - 10.1089/dna.1987.6.255
M3 - Article
C2 - 3109866
AN - SCOPUS:0023222632
VL - 6
SP - 255
EP - 265
JO - DNA and Cell Biology
JF - DNA and Cell Biology
SN - 1044-5498
IS - 3
ER -