Molecular Cloning of an Amylase Gene of Bacillus circulans

Masafumi Nishizawa, Fumiko Ozawa, Fumio Hishinuma

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

An amylase gene of Bacillus circulans was cloned in B. subtilis and its nucleotide sequence was determined. The putative proamylase consists of 528 amino acids, which correspond to a molecular weight of 58,776. Homologous regions with other amylases of Bacillus species were found. A σ55-type promoter is located at about 250 bp upstream from the starting codon. This promoter was also functional in Escherichia coli, and able to express β-galactosidase activity.

Original languageEnglish
Pages (from-to)255-265
Number of pages11
JournalDNA
Volume6
Issue number3
DOIs
Publication statusPublished - 1987 Jun

    Fingerprint

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Nishizawa, M., Ozawa, F., & Hishinuma, F. (1987). Molecular Cloning of an Amylase Gene of Bacillus circulans. DNA, 6(3), 255-265. https://doi.org/10.1089/dna.1987.6.255