Molecular Cloning of an Amylase Gene of Bacillus circulans

Masafumi Nishizawa; Fumiko Ozawa; Fumio Hishinuma

doi:10.1089/dna.1987.6.255

Molecular Cloning of an Amylase Gene of Bacillus circulans

Masafumi Nishizawa, Fumiko Ozawa, Fumio Hishinuma

School of Medicine

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20 Citations (Scopus)

Abstract

An amylase gene of Bacillus circulans was cloned in B. subtilis and its nucleotide sequence was determined. The putative proamylase consists of 528 amino acids, which correspond to a molecular weight of 58,776. Homologous regions with other amylases of Bacillus species were found. A σ⁵⁵-type promoter is located at about 250 bp upstream from the starting codon. This promoter was also functional in Escherichia coli, and able to express β-galactosidase activity.

Original language	English
Pages (from-to)	255-265
Number of pages	11
Journal	DNA
Volume	6
Issue number	3
DOIs	https://doi.org/10.1089/dna.1987.6.255
Publication status	Published - 1987 Jun

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ASJC Scopus subject areas

Biochemistry
Molecular Biology
Genetics

Cite this

Nishizawa, M., Ozawa, F., & Hishinuma, F. (1987). Molecular Cloning of an Amylase Gene of Bacillus circulans. DNA, 6(3), 255-265. https://doi.org/10.1089/dna.1987.6.255

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10.1089/dna.1987.6.255