Molecular cloning of cDNA and genomic DNA for human 25-hydroxyvitamin D3 1α-hydroxylase

Toshiaki Monkawa, Tadashi Yoshida, Shu Wakino, Toshimasa Shinki, Hideharu Anazawa, Hector F. DeLuca, Tatsuo Suda, Matsuhiko Hayashi, Takao Saruta

Research output: Contribution to journalArticle

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Abstract

The 25-hydroxyvitamin D3 1α-hydroxylase (1α-hydroxylase) is a cytochrome P450 enzyme that catalyzes the conversion of 25-hydroxyvitamin D3 to 1α,25-dihydroxyvitamin D3. This enzyme plays an important role in calcium homeostasis. Here we report the molecular cloning of cDNA and gene for human 1α-hydroxylase. The cDNA clone was obtained from a human kidney cDNA library by cross-hybridization with a previously cloned rat cDNA probe. The cDNA consists of 2469 bp and encodes a protein of 508 amino acids that shows 82.5% sequence identity with the rat enzyme. A computer-aided homology search revealed that 1α-hydroxylase shares a relatively high homology with vitamin D3 25-hydroxylase (about 40% amino acid identity). Northern blot analysis showed that the 2.5-kb mRNA is most abundant in kidney. The gene for human 1α-hydroxylase spans approximately 6 kb, is composed of nine exons, and is present as a single copy. This molecular cloning makes it possible to investigate the genetic mechanism of diseases related to calcium metabolism, including vitamin D-dependency rickets type I.

Original languageEnglish
Pages (from-to)527-533
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume239
Issue number2
DOIs
Publication statusPublished - 1997 Oct 20

Fingerprint

Calcifediol
Cloning
Molecular Cloning
Mixed Function Oxygenases
Complementary DNA
DNA
Cytochrome P-450 Enzyme System
Rats
Cholestanetriol 26-Monooxygenase
Genes
Calcium
Kidney
Amino Acids
Rickets
Inborn Genetic Diseases
Calcitriol
Enzymes
Gene Library
Metabolism
Vitamin D

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Molecular cloning of cDNA and genomic DNA for human 25-hydroxyvitamin D3 1α-hydroxylase. / Monkawa, Toshiaki; Yoshida, Tadashi; Wakino, Shu; Shinki, Toshimasa; Anazawa, Hideharu; DeLuca, Hector F.; Suda, Tatsuo; Hayashi, Matsuhiko; Saruta, Takao.

In: Biochemical and Biophysical Research Communications, Vol. 239, No. 2, 20.10.1997, p. 527-533.

Research output: Contribution to journalArticle

Monkawa, Toshiaki ; Yoshida, Tadashi ; Wakino, Shu ; Shinki, Toshimasa ; Anazawa, Hideharu ; DeLuca, Hector F. ; Suda, Tatsuo ; Hayashi, Matsuhiko ; Saruta, Takao. / Molecular cloning of cDNA and genomic DNA for human 25-hydroxyvitamin D3 1α-hydroxylase. In: Biochemical and Biophysical Research Communications. 1997 ; Vol. 239, No. 2. pp. 527-533.
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