Molecular cloning of cDNA and genomic DNA for human 25-hydroxyvitamin D3 1α-hydroxylase

Toshiaki Monkawa, Tadashi Yoshida, Shu Wakino, Toshimasa Shinki, Hideharu Anazawa, Hector F. DeLuca, Tatsuo Suda, Matsuhiko Hayashi, Takao Saruta

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Abstract

The 25-hydroxyvitamin D3 1α-hydroxylase (1α-hydroxylase) is a cytochrome P450 enzyme that catalyzes the conversion of 25-hydroxyvitamin D3 to 1α,25-dihydroxyvitamin D3. This enzyme plays an important role in calcium homeostasis. Here we report the molecular cloning of cDNA and gene for human 1α-hydroxylase. The cDNA clone was obtained from a human kidney cDNA library by cross-hybridization with a previously cloned rat cDNA probe. The cDNA consists of 2469 bp and encodes a protein of 508 amino acids that shows 82.5% sequence identity with the rat enzyme. A computer-aided homology search revealed that 1α-hydroxylase shares a relatively high homology with vitamin D3 25-hydroxylase (about 40% amino acid identity). Northern blot analysis showed that the 2.5-kb mRNA is most abundant in kidney. The gene for human 1α-hydroxylase spans approximately 6 kb, is composed of nine exons, and is present as a single copy. This molecular cloning makes it possible to investigate the genetic mechanism of diseases related to calcium metabolism, including vitamin D-dependency rickets type I.

Original languageEnglish
Pages (from-to)527-533
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume239
Issue number2
DOIs
Publication statusPublished - 1997 Oct 20

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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