Molecular-cloning of cdna encoding human drebrin e and chromosomal mapping of its gene

Masahiro Toda, Tomoaki Shirao, Shinsei Minoshima, Nobuyoshi Shimizu, Shigeo Toya, Keiichi Uyemura

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27 Citations (Scopus)

Abstract

Drebrins are novel actin-binding proteins in the brain which are developmentally regulated. Three isoforms: two embryonic types (El and E2) and an adult type (A) are generated by alternative RNA splicing from a single drebrin gene in the chicken brain. A full length cDNA clone of human drebrin E has been isolated from a cDNA library of human fetus brain. The clone is 2596 base pairs in length and contains an open reading frame of 1947 nucleotides encoding a protein of 649 amino acids. The deduced amino acid sequence, except for the internal 138-nucleotide sequence (ins2), exhibits 88% homology with rat drebrin A. Spot blot hybridization using flow-sorted human chromosomes provides evidence that the gene encoding human drebrin protein locates on human chromosome 5.

Original languageEnglish
Pages (from-to)468-472
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume196
Issue number1
DOIs
Publication statusPublished - 1993 Oct 15

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ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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