Molecular cloning of cDNA encoding human Rab3D whose expression is upregulated with myeloid differentiation

Hitoshi Nishio, Toshio Suda, Ken Ichi Sawada, Takeshi Miyamoto, Takao Koike, Yuji Yamaguchi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To identify genes expressed in myeloid differentiation, we isolated a cDNA fragment by differential display using RNA prepared from HT93A cells, a human cell line capable of differentiating into neutrophil and eosinophil lineages in response to retinoic acid (RA). Evaluation of the full-length clone isolated from an HT93A cDNA library showed that it encoded a 24 kDa protein comprised of several domains conserved in the Ras superfamily. Comparison of the deduced amino acid sequence of this clone with Rab proteins revealed that it had highest homology to a small GTP-binding protein, murine Rab3D. The mRNA expression of human Rab3D was upregulated in the course of myeloid differentiation, and it was preferentially expressed in granulocytes. These results suggest that human Rab3D may play a specific role in granulocytes, for example in exocytosis of neutrophil-specific granules or in degranulation of both eosinophils and basophils.

Original languageEnglish
Pages (from-to)283-290
Number of pages8
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1444
Issue number2
DOIs
Publication statusPublished - 1999 Feb 16
Externally publishedYes

Fingerprint

Cloning
Molecular Cloning
Complementary DNA
Granulocytes
Eosinophils
Neutrophils
Clone Cells
Tretinoin
Gene Library
GTP-Binding Proteins
Proteins
Basophils
Genes
Display devices
Cells
Exocytosis
RNA
Amino Acids
Messenger RNA
Amino Acid Sequence

Keywords

  • cDNA cloning
  • In vitro transcription/translation
  • mRNA expression
  • Myeloid differentiation
  • Polymerase chain reaction differential display
  • Rab3D

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

Molecular cloning of cDNA encoding human Rab3D whose expression is upregulated with myeloid differentiation. / Nishio, Hitoshi; Suda, Toshio; Sawada, Ken Ichi; Miyamoto, Takeshi; Koike, Takao; Yamaguchi, Yuji.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1444, No. 2, 16.02.1999, p. 283-290.

Research output: Contribution to journalArticle

Nishio, Hitoshi ; Suda, Toshio ; Sawada, Ken Ichi ; Miyamoto, Takeshi ; Koike, Takao ; Yamaguchi, Yuji. / Molecular cloning of cDNA encoding human Rab3D whose expression is upregulated with myeloid differentiation. In: Biochimica et Biophysica Acta - Gene Structure and Expression. 1999 ; Vol. 1444, No. 2. pp. 283-290.
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