Molecular cloning of hamster brain and atrial natriuretic peptide cDNAs: Cardiomyopathic hamsters are useful models for brain and atrial natriuretic peptides

Naohisa Tamura, Yoshihiro Ogawa, Hiroshi Itoh, Hiroshi Arai, Shin Ichi Suga, Osamu Nakagawa, Yasato Komatsu, Ichiro Kishimoto, Kazuhiko Takaya, Takaaki Yoshimasa, Shozo Shiono, Kazuwa Nakao

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Brain and atrial natriuretic peptides (BNP and ANP) are cardiac hormones with diuretic, natriuretic, and vasodilatory activities. Cardiomyopathic hamsters are widely used animal models of heart failure. Due to the structural divergence of BNP among species, examination on pathophysiological roles of BNP using cardiomyopathic hamsters is so far impossible. We therefore isolated hamster BNP and ANP cDNAs, and investigated synthesis and secretion of these peptides in normal and cardiomyopathic hamsters. The COOH-terminal 32-residue peptide of cloned hamster pre-proBNP with 122 amino acids, preceded by a single arginine residue, supposedly represents hamster BNP showing < 50% homology to rat BNP. Alpha-hamster ANP, 28-residue peptide, is identical to α-rat ANP. In hamsters, BNP and ANP occur mainly in the ventricle and the atrium, respectively. The 32-wk-old hypertrophic cardiomyopathic BIO14.6 strain exhibited ventricular hypertrophy. The 32-wk-old dilated cardiomyopathic BIO53.58 strain remained at the stage without apparent heart failure. In BIO14.6 and BIO53.58 strains at this age, ventricular BNP and ANP gene expressions are augmented, and the plasma BNP concentration is elevated to 136 and 108 fmol/ml, respectively, three times greater than the elevated plasma ANP concentration, which well mimics changes of the plasma BNP and ANP concentrations in human heart failure. Cardiomyopathic hamsters, therefore, are useful models to investigate the implication of BNP in human cardiovascular diseases.

Original languageEnglish
Pages (from-to)1059-1068
Number of pages10
JournalJournal of Clinical Investigation
Volume94
Issue number3
Publication statusPublished - 1994 Sep
Externally publishedYes

Fingerprint

Brain Natriuretic Peptide
Molecular Cloning
Atrial Natriuretic Factor
Cricetinae
Complementary DNA
Heart Failure
Peptides
Diuretics
Hypertrophy
Arginine
Cardiovascular Diseases
Animal Models
Hormones
Gene Expression
Amino Acids

Keywords

  • Cardiac hypertrophy
  • Congestive heart failure
  • Gene expression
  • Radioimmunoassay
  • Tissue distribution

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Molecular cloning of hamster brain and atrial natriuretic peptide cDNAs : Cardiomyopathic hamsters are useful models for brain and atrial natriuretic peptides. / Tamura, Naohisa; Ogawa, Yoshihiro; Itoh, Hiroshi; Arai, Hiroshi; Suga, Shin Ichi; Nakagawa, Osamu; Komatsu, Yasato; Kishimoto, Ichiro; Takaya, Kazuhiko; Yoshimasa, Takaaki; Shiono, Shozo; Nakao, Kazuwa.

In: Journal of Clinical Investigation, Vol. 94, No. 3, 09.1994, p. 1059-1068.

Research output: Contribution to journalArticle

Tamura, N, Ogawa, Y, Itoh, H, Arai, H, Suga, SI, Nakagawa, O, Komatsu, Y, Kishimoto, I, Takaya, K, Yoshimasa, T, Shiono, S & Nakao, K 1994, 'Molecular cloning of hamster brain and atrial natriuretic peptide cDNAs: Cardiomyopathic hamsters are useful models for brain and atrial natriuretic peptides', Journal of Clinical Investigation, vol. 94, no. 3, pp. 1059-1068.
Tamura, Naohisa ; Ogawa, Yoshihiro ; Itoh, Hiroshi ; Arai, Hiroshi ; Suga, Shin Ichi ; Nakagawa, Osamu ; Komatsu, Yasato ; Kishimoto, Ichiro ; Takaya, Kazuhiko ; Yoshimasa, Takaaki ; Shiono, Shozo ; Nakao, Kazuwa. / Molecular cloning of hamster brain and atrial natriuretic peptide cDNAs : Cardiomyopathic hamsters are useful models for brain and atrial natriuretic peptides. In: Journal of Clinical Investigation. 1994 ; Vol. 94, No. 3. pp. 1059-1068.
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abstract = "Brain and atrial natriuretic peptides (BNP and ANP) are cardiac hormones with diuretic, natriuretic, and vasodilatory activities. Cardiomyopathic hamsters are widely used animal models of heart failure. Due to the structural divergence of BNP among species, examination on pathophysiological roles of BNP using cardiomyopathic hamsters is so far impossible. We therefore isolated hamster BNP and ANP cDNAs, and investigated synthesis and secretion of these peptides in normal and cardiomyopathic hamsters. The COOH-terminal 32-residue peptide of cloned hamster pre-proBNP with 122 amino acids, preceded by a single arginine residue, supposedly represents hamster BNP showing < 50{\%} homology to rat BNP. Alpha-hamster ANP, 28-residue peptide, is identical to α-rat ANP. In hamsters, BNP and ANP occur mainly in the ventricle and the atrium, respectively. The 32-wk-old hypertrophic cardiomyopathic BIO14.6 strain exhibited ventricular hypertrophy. The 32-wk-old dilated cardiomyopathic BIO53.58 strain remained at the stage without apparent heart failure. In BIO14.6 and BIO53.58 strains at this age, ventricular BNP and ANP gene expressions are augmented, and the plasma BNP concentration is elevated to 136 and 108 fmol/ml, respectively, three times greater than the elevated plasma ANP concentration, which well mimics changes of the plasma BNP and ANP concentrations in human heart failure. Cardiomyopathic hamsters, therefore, are useful models to investigate the implication of BNP in human cardiovascular diseases.",
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AU - Ogawa, Yoshihiro

AU - Itoh, Hiroshi

AU - Arai, Hiroshi

AU - Suga, Shin Ichi

AU - Nakagawa, Osamu

AU - Komatsu, Yasato

AU - Kishimoto, Ichiro

AU - Takaya, Kazuhiko

AU - Yoshimasa, Takaaki

AU - Shiono, Shozo

AU - Nakao, Kazuwa

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KW - Tissue distribution

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