TY - JOUR
T1 - Molecular cloning of rat macrophage-stimulating protein and its involvement in the male reproductive system
AU - Ohshiro, Kazuiku
AU - Iwama, Atsushi
AU - Matsuno, Kenjiro
AU - Ezaki, Taichi
AU - Sakamoto, Osamu
AU - Hamaguchi, Isao
AU - Takasu, Nobuyuki
AU - Suda, Toshio
N1 - Funding Information:
We thank Drs. Edward J. Leonard and Ming-Hai Wang (National Cancer Institute, Frederick Cancer Research and Development Center) for providing the rabbit anti-human MSP polyclonal antibody, and Dr. Kazuo Maruyama (Tokyo Medical and Dental University) for the MKITNeo expression vector. This work was supported by grants-in-aid from the Ministry of Education, Science and Culture of Japan.
PY - 1996/10/3
Y1 - 1996/10/3
N2 - Macrophage-stimulating protein (MSP), a member of the hepatocyte growth factor family, is a ligand for receptor tyrosine kinase STK/RON. Here we isolated a full-length cDNA of rat MSP and a partial cDNA of rat STK/RON, then characterized their expression in the male reproductive system. In situ hybridization revealed that MSP mRNA was localized to spermatogonia and spermatocytes in the testis and the epithelium lining the lumen of the epididymis. On the other hand, RT-PCR analysis showed that STK/RON mRNA was expressed in sperm collected from both testis and epididymis. These findings suggest that locally produced MSP may play a vital role in germ cell-germ cell interaction during spermatogenesis as well as in the acquisition of sperm motility and/or fertilizing capacity in the epididymis. Our findings reveal new possible roles of the MSP-STK/RON signaling pathway.
AB - Macrophage-stimulating protein (MSP), a member of the hepatocyte growth factor family, is a ligand for receptor tyrosine kinase STK/RON. Here we isolated a full-length cDNA of rat MSP and a partial cDNA of rat STK/RON, then characterized their expression in the male reproductive system. In situ hybridization revealed that MSP mRNA was localized to spermatogonia and spermatocytes in the testis and the epithelium lining the lumen of the epididymis. On the other hand, RT-PCR analysis showed that STK/RON mRNA was expressed in sperm collected from both testis and epididymis. These findings suggest that locally produced MSP may play a vital role in germ cell-germ cell interaction during spermatogenesis as well as in the acquisition of sperm motility and/or fertilizing capacity in the epididymis. Our findings reveal new possible roles of the MSP-STK/RON signaling pathway.
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U2 - 10.1006/bbrc.1996.1500
DO - 10.1006/bbrc.1996.1500
M3 - Article
C2 - 8858136
AN - SCOPUS:0030567977
SN - 0006-291X
VL - 227
SP - 273
EP - 280
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -