Molecular emm genotyping and antibiotic susceptibility of Streptococcus dysgalactiae subsp. equisimilis isolated from invasive and non-invasive infections

Katsuhiko Sunaoshi, Somay Y. Murayama, Keiko Adachi, Michiko Yagoshi, Katsuko Okuzumi, Naoko Chiba, Miyuki Morozumi, Kimiko Ubukata

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

To analyse the characteristics of infections caused by Streptococcus dysgalactiae subsp. equisimilis, clinical isolates (n=145) were collected at 11 medical institutions between September 2003 and October 2005. These isolates belonged to Lancefield group A (n=5), group C (n=18) or group G (n=122). Among all isolates, 42 strains were isolated from sterile samples such as blood, synovial fluid and tissue specimens from patients who were mostly over 50 years with invasive infections, and included seven cases of streptococcal toxic shock syndrome and necrotizing fasciitis. In contrast, the remaining 103 were isolated mainly from patients of all age groups with non-invasive infections such as pharyngotonsillitis. These isolates were classified into 25 types based on emm genotyping. A significant difference in emm types was observed between isolates from invasive and non-invasive infections (P<0.001): stG485, stG6792 and stG2078 predominated among isolates from invasive infections. A phylogenetic tree of complete open reading frames of emm genes in this organism showed high homology with those of Streptococcus pyogenes, but not with those of other streptococci. The presence of five different clones was estimated based on DNA profiles of isolates from invasive infections obtained by PFGE. Genes for resistance to macrolides [erm(A), three isolates; erm(B), five isolates; mef(A), seven isolates] and levofloxacin (mutations in gyrA and parC, four isolates) were identified in this organism. These results suggest the need for further nationwide surveillance of invasive infections caused by S. dysgalactiae subsp. equisimilis.

Original languageEnglish
Pages (from-to)82-88
Number of pages7
JournalJournal of Medical Microbiology
Volume59
Issue number1
DOIs
Publication statusPublished - 2010 Jan
Externally publishedYes

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Streptococcus
Anti-Bacterial Agents
Infection
Necrotizing Fasciitis
Levofloxacin
Streptococcus pyogenes
Synovial Fluid
Macrolides
Septic Shock
Open Reading Frames
Genes
Clone Cells
Age Groups
Mutation
DNA

ASJC Scopus subject areas

  • Microbiology (medical)
  • Microbiology

Cite this

Molecular emm genotyping and antibiotic susceptibility of Streptococcus dysgalactiae subsp. equisimilis isolated from invasive and non-invasive infections. / Sunaoshi, Katsuhiko; Murayama, Somay Y.; Adachi, Keiko; Yagoshi, Michiko; Okuzumi, Katsuko; Chiba, Naoko; Morozumi, Miyuki; Ubukata, Kimiko.

In: Journal of Medical Microbiology, Vol. 59, No. 1, 01.2010, p. 82-88.

Research output: Contribution to journalArticle

Sunaoshi, Katsuhiko ; Murayama, Somay Y. ; Adachi, Keiko ; Yagoshi, Michiko ; Okuzumi, Katsuko ; Chiba, Naoko ; Morozumi, Miyuki ; Ubukata, Kimiko. / Molecular emm genotyping and antibiotic susceptibility of Streptococcus dysgalactiae subsp. equisimilis isolated from invasive and non-invasive infections. In: Journal of Medical Microbiology. 2010 ; Vol. 59, No. 1. pp. 82-88.
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