Molecular factors that are associated with early developmental arrest of intraerythrocytic Plasmodium falciparum

Hiroko Asahi, Mohammed Essa Marghany Tolba, Masanobu Tanabe, Hiroshi Ohmae

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Malaria continues to be a devastating disease. We investigated the factors that control intraerythrocytic development of the parasite Plasmodium falciparum by using a chemically defined medium (CDM) containing non-esterified fatty acid(s) (NEFA) and phospholipids with specific fatty acid moieties, to identify substances crucial for parasite development. Different NEFAs in the CDM played distinct roles by altering the development of the parasite at various stages, with effects ranging from complete growth to growth arrest at the ring stage. We used genome-wide transcriptome profiling to identify genes that were differentially expressed among the different developmental stages of the parasite, cultured in the presence of various NEFAs. We predicted 26 transcripts that were associated with the suppression of schizogony, of which 5 transcripts, including merozoite surface protein 2, a putative DEAD/DEAH box RNA helicase, serine repeat antigen 3, a putative copper channel, and palmitoyl acyltransferase, were particularly associated with blockage of trophozoite progression from the ring stage. Furthermore, the involvement of copper ions in developmental arrest was detected by copper-ion-chelating methods, implying a critical function of copper homeostasis in the early growth stage of the parasite. These results should help to elucidate the mechanisms behind the development of P. falciparum.

Original languageEnglish
Pages (from-to)485-493
Number of pages9
JournalCanadian Journal of Microbiology
Volume59
Issue number7
DOIs
Publication statusPublished - 2013

Fingerprint

Plasmodium falciparum
Parasites
Copper
Nonesterified Fatty Acids
Fatty Acids
DEAD-box RNA Helicases
Growth
Ions
Merozoites
Acyltransferases
Trophozoites
Gene Expression Profiling
Serine
Malaria
Phospholipids
Membrane Proteins
Homeostasis
Genome
Antigens
Genes

Keywords

  • Chemically defined culture medium
  • Copper homeostasis
  • Developmental arrest
  • Genome-wide transcriptome profiling
  • Plasmodium falciparum

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Immunology
  • Genetics
  • Molecular Biology

Cite this

Molecular factors that are associated with early developmental arrest of intraerythrocytic Plasmodium falciparum. / Asahi, Hiroko; Tolba, Mohammed Essa Marghany; Tanabe, Masanobu; Ohmae, Hiroshi.

In: Canadian Journal of Microbiology, Vol. 59, No. 7, 2013, p. 485-493.

Research output: Contribution to journalArticle

Asahi, Hiroko ; Tolba, Mohammed Essa Marghany ; Tanabe, Masanobu ; Ohmae, Hiroshi. / Molecular factors that are associated with early developmental arrest of intraerythrocytic Plasmodium falciparum. In: Canadian Journal of Microbiology. 2013 ; Vol. 59, No. 7. pp. 485-493.
@article{6cf005b649cf42e2a9e488506b8108ba,
title = "Molecular factors that are associated with early developmental arrest of intraerythrocytic Plasmodium falciparum",
abstract = "Malaria continues to be a devastating disease. We investigated the factors that control intraerythrocytic development of the parasite Plasmodium falciparum by using a chemically defined medium (CDM) containing non-esterified fatty acid(s) (NEFA) and phospholipids with specific fatty acid moieties, to identify substances crucial for parasite development. Different NEFAs in the CDM played distinct roles by altering the development of the parasite at various stages, with effects ranging from complete growth to growth arrest at the ring stage. We used genome-wide transcriptome profiling to identify genes that were differentially expressed among the different developmental stages of the parasite, cultured in the presence of various NEFAs. We predicted 26 transcripts that were associated with the suppression of schizogony, of which 5 transcripts, including merozoite surface protein 2, a putative DEAD/DEAH box RNA helicase, serine repeat antigen 3, a putative copper channel, and palmitoyl acyltransferase, were particularly associated with blockage of trophozoite progression from the ring stage. Furthermore, the involvement of copper ions in developmental arrest was detected by copper-ion-chelating methods, implying a critical function of copper homeostasis in the early growth stage of the parasite. These results should help to elucidate the mechanisms behind the development of P. falciparum.",
keywords = "Chemically defined culture medium, Copper homeostasis, Developmental arrest, Genome-wide transcriptome profiling, Plasmodium falciparum",
author = "Hiroko Asahi and Tolba, {Mohammed Essa Marghany} and Masanobu Tanabe and Hiroshi Ohmae",
year = "2013",
doi = "10.1139/cjm-2013-0166",
language = "English",
volume = "59",
pages = "485--493",
journal = "Canadian Journal of Microbiology",
issn = "0008-4166",
publisher = "National Research Council of Canada",
number = "7",

}

TY - JOUR

T1 - Molecular factors that are associated with early developmental arrest of intraerythrocytic Plasmodium falciparum

AU - Asahi, Hiroko

AU - Tolba, Mohammed Essa Marghany

AU - Tanabe, Masanobu

AU - Ohmae, Hiroshi

PY - 2013

Y1 - 2013

N2 - Malaria continues to be a devastating disease. We investigated the factors that control intraerythrocytic development of the parasite Plasmodium falciparum by using a chemically defined medium (CDM) containing non-esterified fatty acid(s) (NEFA) and phospholipids with specific fatty acid moieties, to identify substances crucial for parasite development. Different NEFAs in the CDM played distinct roles by altering the development of the parasite at various stages, with effects ranging from complete growth to growth arrest at the ring stage. We used genome-wide transcriptome profiling to identify genes that were differentially expressed among the different developmental stages of the parasite, cultured in the presence of various NEFAs. We predicted 26 transcripts that were associated with the suppression of schizogony, of which 5 transcripts, including merozoite surface protein 2, a putative DEAD/DEAH box RNA helicase, serine repeat antigen 3, a putative copper channel, and palmitoyl acyltransferase, were particularly associated with blockage of trophozoite progression from the ring stage. Furthermore, the involvement of copper ions in developmental arrest was detected by copper-ion-chelating methods, implying a critical function of copper homeostasis in the early growth stage of the parasite. These results should help to elucidate the mechanisms behind the development of P. falciparum.

AB - Malaria continues to be a devastating disease. We investigated the factors that control intraerythrocytic development of the parasite Plasmodium falciparum by using a chemically defined medium (CDM) containing non-esterified fatty acid(s) (NEFA) and phospholipids with specific fatty acid moieties, to identify substances crucial for parasite development. Different NEFAs in the CDM played distinct roles by altering the development of the parasite at various stages, with effects ranging from complete growth to growth arrest at the ring stage. We used genome-wide transcriptome profiling to identify genes that were differentially expressed among the different developmental stages of the parasite, cultured in the presence of various NEFAs. We predicted 26 transcripts that were associated with the suppression of schizogony, of which 5 transcripts, including merozoite surface protein 2, a putative DEAD/DEAH box RNA helicase, serine repeat antigen 3, a putative copper channel, and palmitoyl acyltransferase, were particularly associated with blockage of trophozoite progression from the ring stage. Furthermore, the involvement of copper ions in developmental arrest was detected by copper-ion-chelating methods, implying a critical function of copper homeostasis in the early growth stage of the parasite. These results should help to elucidate the mechanisms behind the development of P. falciparum.

KW - Chemically defined culture medium

KW - Copper homeostasis

KW - Developmental arrest

KW - Genome-wide transcriptome profiling

KW - Plasmodium falciparum

UR - http://www.scopus.com/inward/record.url?scp=84879895217&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84879895217&partnerID=8YFLogxK

U2 - 10.1139/cjm-2013-0166

DO - 10.1139/cjm-2013-0166

M3 - Article

VL - 59

SP - 485

EP - 493

JO - Canadian Journal of Microbiology

JF - Canadian Journal of Microbiology

SN - 0008-4166

IS - 7

ER -