Molecular Genetic Analysis of Myelin‐Deficient Mice: Shiverer Mutant Mice Show Deletion in Gene(s) Coding for Myelin Basic Protein

Minoru Kimura, Hidetoshi Inoko, Motoya Katsuki, Asako Ando, Tadayuki Sato, Tadaaki Hirose, Hideaki Takashima, Seiichi Inayama, Hideyuki Okano, Ken Takamatsu, Katsuhiko Mikoshiba, Yasuzo Tsukada, Itaru Watanabe

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Abstract: The gene expression of myelin basic proteins (MBPs) in shiverer mutant mice was investigated by the Northern and Southern hybridization techniques. In the control mice RNA molecules from the brains which were about 2,300 nucleotides in length were hybridized to cDNA of 1.8 kb encoding for a mouse MBP, but RNA from the brains of 3‐week‐old shiverer mutant mice contained no detectable amount of MBP transcripts hybridizing to this probe. Moreover the shiverer mutant mice lost several restriction fragments that hybridized to the same probe in the control mice when each of the five restriction enzymes, i.e., HindIII, PstI, PvuII, AccI, and StuI, was used. These data suggest that the shiverer mutation may correspond to the deletion of a large portion of MBP exon(s) in the gene, and this deletion causes inefficient transcription leading to the depletion of MBPs in the myelin and the dysmyelination observed in these mice.

Original languageEnglish
Pages (from-to)692-696
Number of pages5
JournalJournal of Neurochemistry
Volume44
Issue number3
DOIs
Publication statusPublished - 1985 Mar

Keywords

  • Deletion mutant
  • Dysmyelination
  • Myelin basic protein
  • Northern blotting analysis
  • Shiverer mouse
  • Southern blotting analysis

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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    Kimura, M., Inoko, H., Katsuki, M., Ando, A., Sato, T., Hirose, T., Takashima, H., Inayama, S., Okano, H., Takamatsu, K., Mikoshiba, K., Tsukada, Y., & Watanabe, I. (1985). Molecular Genetic Analysis of Myelin‐Deficient Mice: Shiverer Mutant Mice Show Deletion in Gene(s) Coding for Myelin Basic Protein. Journal of Neurochemistry, 44(3), 692-696. https://doi.org/10.1111/j.1471-4159.1985.tb12870.x