Molecular mass measurement of polymerase chain reaction products amplified from human blood dna by electrospray ionization mass spectrometry

Yasuhiro Naito, Keiichiro Ishikawa, Yoshinori Koga, Toshihiro Tsuneyoshi, Hideya Terunuma, Ryuichi Arakawa

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)

Abstract

We report here on the first analysis of polymerase chain reaction (PCR) products amplified from a small amount of human blood DNA by electrospray ionization mass spectrometry (ESI‐MS). Adenomatous polyposis coli (APC) gene fragment of about 50 base pairs (bp) with a relative molecular mass (Mr) of approximately 15 000 u was amplified from human blood DNA by PCR. The accurate molecular mass of the PCR products was determined with an accuracy of ∼0.005% by ESI‐MS. The amount of DNA used was only 100 ng (∼ 50 zmol; the theoretically required amount of blood is therefore less than 1 μL for PCR). The ESI‐MS measurement of the PCR products proved to be a new accurate, sensitive and fast tool for gene diagnosis.

Original languageEnglish
Pages (from-to)1484-1486
Number of pages3
JournalRapid Communications in Mass Spectrometry
Volume9
Issue number15
DOIs
Publication statusPublished - 1995
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry

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