Molecular mechanism of adaptive cytoprotection induced by ethanol in human gastric cells

Shinji Tsutsumi, Shinji Mima, Wataru Tomisato, Tatsuya Hoshino, Tomufusa Tsuchiya, Tohru Mizushima

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Adaptive cytoprotection is the process by which the pretreatment of cells with low concentrations of a noxious agent prevents the damage caused by a subsequent exposure of those cells to higher concentrations of that same agent. In this study, a human gastric carcinoma cell line was used to examine the molecular mechanism of adaptive cytoprotection induced by ethanol. Pretreatment of cells with 1%-4% ethanol made cells resistant to a subsequent exposure to 8% ethanol. This adaptive cytoprotection was accompanied by an increase in prostaglandin E2 synthesis and was partially inhibited by inhibitors of cyclooxygenase-2, but not by an inhibitor of cyclooxygenase-1. Furthermore, the adaptive cytoprotection was not dependent on newly synthesized proteins and was inhibited by a protein tyrosine kinase inhibitor. Based on these results, it is proposed that the stimulation of cyclooxygenase-2-dependent prostaglandin E2 synthesis, which is regulated post-translationally by protein tyrosine phosphorylation, plays an important role in adaptive cytoprotection induced by ethanol in gastric cells.

Original languageEnglish
Pages (from-to)1089-1095
Number of pages7
JournalExperimental Biology and Medicine
Volume228
Issue number9
Publication statusPublished - 2003 Oct
Externally publishedYes

Fingerprint

Cytoprotection
Stomach
Ethanol
Cells
Dinoprostone
Cyclooxygenase 1
Phosphorylation
Cyclooxygenase 2 Inhibitors
Cyclooxygenase 2
Protein-Tyrosine Kinases
Tyrosine
Protein Kinase Inhibitors
Proteins
Carcinoma
Cell Line

Keywords

  • Post-translational regulation
  • Prostaglandin E
  • Protein tyrosine phosphorylation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Tsutsumi, S., Mima, S., Tomisato, W., Hoshino, T., Tsuchiya, T., & Mizushima, T. (2003). Molecular mechanism of adaptive cytoprotection induced by ethanol in human gastric cells. Experimental Biology and Medicine, 228(9), 1089-1095.

Molecular mechanism of adaptive cytoprotection induced by ethanol in human gastric cells. / Tsutsumi, Shinji; Mima, Shinji; Tomisato, Wataru; Hoshino, Tatsuya; Tsuchiya, Tomufusa; Mizushima, Tohru.

In: Experimental Biology and Medicine, Vol. 228, No. 9, 10.2003, p. 1089-1095.

Research output: Contribution to journalArticle

Tsutsumi, S, Mima, S, Tomisato, W, Hoshino, T, Tsuchiya, T & Mizushima, T 2003, 'Molecular mechanism of adaptive cytoprotection induced by ethanol in human gastric cells', Experimental Biology and Medicine, vol. 228, no. 9, pp. 1089-1095.
Tsutsumi S, Mima S, Tomisato W, Hoshino T, Tsuchiya T, Mizushima T. Molecular mechanism of adaptive cytoprotection induced by ethanol in human gastric cells. Experimental Biology and Medicine. 2003 Oct;228(9):1089-1095.
Tsutsumi, Shinji ; Mima, Shinji ; Tomisato, Wataru ; Hoshino, Tatsuya ; Tsuchiya, Tomufusa ; Mizushima, Tohru. / Molecular mechanism of adaptive cytoprotection induced by ethanol in human gastric cells. In: Experimental Biology and Medicine. 2003 ; Vol. 228, No. 9. pp. 1089-1095.
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