Abstract
Adaptive cytoprotection is the process by which the pretreatment of cells with low concentrations of a noxious agent prevents the damage caused by a subsequent exposure of those cells to higher concentrations of that same agent. In this study, a human gastric carcinoma cell line was used to examine the molecular mechanism of adaptive cytoprotection induced by ethanol. Pretreatment of cells with 1%-4% ethanol made cells resistant to a subsequent exposure to 8% ethanol. This adaptive cytoprotection was accompanied by an increase in prostaglandin E2 synthesis and was partially inhibited by inhibitors of cyclooxygenase-2, but not by an inhibitor of cyclooxygenase-1. Furthermore, the adaptive cytoprotection was not dependent on newly synthesized proteins and was inhibited by a protein tyrosine kinase inhibitor. Based on these results, it is proposed that the stimulation of cyclooxygenase-2-dependent prostaglandin E2 synthesis, which is regulated post-translationally by protein tyrosine phosphorylation, plays an important role in adaptive cytoprotection induced by ethanol in gastric cells.
| Original language | English |
|---|---|
| Pages (from-to) | 1089-1095 |
| Number of pages | 7 |
| Journal | Experimental Biology and Medicine |
| Volume | 228 |
| Issue number | 9 |
| Publication status | Published - 2003 Oct |
| Externally published | Yes |
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Keywords
- Post-translational regulation
- Prostaglandin E
- Protein tyrosine phosphorylation
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Molecular mechanism of adaptive cytoprotection induced by ethanol in human gastric cells. / Tsutsumi, Shinji; Mima, Shinji; Tomisato, Wataru; Hoshino, Tatsuya; Tsuchiya, Tomufusa; Mizushima, Tohru.
In: Experimental Biology and Medicine, Vol. 228, No. 9, 10.2003, p. 1089-1095.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Molecular mechanism of adaptive cytoprotection induced by ethanol in human gastric cells
AU - Tsutsumi, Shinji
AU - Mima, Shinji
AU - Tomisato, Wataru
AU - Hoshino, Tatsuya
AU - Tsuchiya, Tomufusa
AU - Mizushima, Tohru
PY - 2003/10
Y1 - 2003/10
N2 - Adaptive cytoprotection is the process by which the pretreatment of cells with low concentrations of a noxious agent prevents the damage caused by a subsequent exposure of those cells to higher concentrations of that same agent. In this study, a human gastric carcinoma cell line was used to examine the molecular mechanism of adaptive cytoprotection induced by ethanol. Pretreatment of cells with 1%-4% ethanol made cells resistant to a subsequent exposure to 8% ethanol. This adaptive cytoprotection was accompanied by an increase in prostaglandin E2 synthesis and was partially inhibited by inhibitors of cyclooxygenase-2, but not by an inhibitor of cyclooxygenase-1. Furthermore, the adaptive cytoprotection was not dependent on newly synthesized proteins and was inhibited by a protein tyrosine kinase inhibitor. Based on these results, it is proposed that the stimulation of cyclooxygenase-2-dependent prostaglandin E2 synthesis, which is regulated post-translationally by protein tyrosine phosphorylation, plays an important role in adaptive cytoprotection induced by ethanol in gastric cells.
AB - Adaptive cytoprotection is the process by which the pretreatment of cells with low concentrations of a noxious agent prevents the damage caused by a subsequent exposure of those cells to higher concentrations of that same agent. In this study, a human gastric carcinoma cell line was used to examine the molecular mechanism of adaptive cytoprotection induced by ethanol. Pretreatment of cells with 1%-4% ethanol made cells resistant to a subsequent exposure to 8% ethanol. This adaptive cytoprotection was accompanied by an increase in prostaglandin E2 synthesis and was partially inhibited by inhibitors of cyclooxygenase-2, but not by an inhibitor of cyclooxygenase-1. Furthermore, the adaptive cytoprotection was not dependent on newly synthesized proteins and was inhibited by a protein tyrosine kinase inhibitor. Based on these results, it is proposed that the stimulation of cyclooxygenase-2-dependent prostaglandin E2 synthesis, which is regulated post-translationally by protein tyrosine phosphorylation, plays an important role in adaptive cytoprotection induced by ethanol in gastric cells.
KW - Post-translational regulation
KW - Prostaglandin E
KW - Protein tyrosine phosphorylation
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UR - http://www.scopus.com/inward/citedby.url?scp=0141640845&partnerID=8YFLogxK
M3 - Article
C2 - 14530521
AN - SCOPUS:0141640845
VL - 228
SP - 1089
EP - 1095
JO - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
JF - Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N. Y.)
SN - 0037-9727
IS - 9
ER -