Molecular mechanisms regulating dissociation of cell-cell junction of epithelial cells by oxidative stress

Junko Inumaru, Osamu Nagano, Eri Takahashi, Takatsugu Ishimoto, Satoshi Nakamura, Yoshimi Suzuki, Shin Ichiro Niwa, Kazuo Umezawa, Hidenobu Tanihara, Hideyuki Saya

Research output: Contribution to journalArticlepeer-review

64 Citations (Scopus)


Oxidative stress is regarded as a causative factor in aging and various degenerative diseases. Here, we show the mechanism by which oxidative stress induces disruption of cell-cell junctions using retinal pigment epithelial cells. We demonstrated that reactive oxygen species (ROS)-mediated activation of Src kinase increases the tyrosine phosphorylation state of p120-catenin and rapidly triggers translocation of p120-catenin and internalization of N-cadherin from the cell-cell adhesion sites to an early endosomal compartment. Endosomal accumulation of p120-catenin resulted in stress fiber formation and cell-cell dissociation through the activation of Rho/Rho kinase pathway. However, these cytoskeletal remodeling and cell-cell dissociation induced by oxidative stress were transient, due to the activation of nuclear factor-κB (NF-κB) and the expression of manganese superoxide dismutase (Mn-SOD). Using the NF-κB specific inhibitor DHMEQ, we found that NF-κB is part of a negative feedback loop to control intracellular ROS levels. Finally, we demonstrated that H2 O2 treatment alone does not induce the epithelial mesenchymal transition (EMT) in retinal pigment epithelial cells, which can be induced by TNF-α treatment. These findings suggest that oxidative stress is a crucial factor to induce the cell-cell dissociation, an initial step of EMT, but does not provide sufficient signals to establish and to maintain the EMT.

Original languageEnglish
Pages (from-to)703-716
Number of pages14
JournalGenes to Cells
Issue number6
Publication statusPublished - 2009 Jun 15

ASJC Scopus subject areas

  • Genetics
  • Cell Biology


Dive into the research topics of 'Molecular mechanisms regulating dissociation of cell-cell junction of epithelial cells by oxidative stress'. Together they form a unique fingerprint.

Cite this