Molecular properties of TAR DNA binding protein-43 fragments are dependent upon its cleavage site

Yoshiaki Furukawa, Kumi Kaneko, Nobuyuki Nukina

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Aggregation of TAR DNA binding protein-43 (TDP-43) is a hallmark feature of amyotrophic lateral sclerosis and frontotemporal lobar degeneration. Under pathogenic conditions, abnormal cleavage of TDP-43 produces the phosphorylated C-terminal fragments (CTFs), which are enriched in neuronal inclusions; however, molecular properties of those TDP-43 fragments remain to be characterized. Here we show distinct degrees of solubility and phosphorylation among fragments truncated at different sites of TDP-43. Truncations were tested mainly within a second RNA recognition motif (RRM2) of TDP-43; when the truncation site was more C-terminal in an RRM2 domain, a TDP-43 CTF basically became less soluble and more phosphorylated in differentiated Neuro2a cells. We also found that cleavage at the third β-strand in RRM2 leads to the formation of SDS-resistant soluble oligomers. Molecular properties of TDP-43 fragments thus significantly depend upon its cleavage site, which might reflect distinct molecular pathologies among sub-types of TDP-43 proteinopathies.

Original languageEnglish
Pages (from-to)1577-1583
Number of pages7
JournalBiochimica et Biophysica Acta - Molecular Basis of Disease
Volume1812
Issue number12
DOIs
Publication statusPublished - 2011 Dec

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DNA-Binding Proteins
Frontotemporal Lobar Degeneration
Molecular Pathology
Amyotrophic Lateral Sclerosis
Protein C
Solubility
Phosphorylation

Keywords

  • Amyotrophic lateral sclerosis
  • Frontotemporal lobar degeneration
  • Protein aggregation
  • TAR DNA binding protein-43

ASJC Scopus subject areas

  • Molecular Biology
  • Molecular Medicine

Cite this

Molecular properties of TAR DNA binding protein-43 fragments are dependent upon its cleavage site. / Furukawa, Yoshiaki; Kaneko, Kumi; Nukina, Nobuyuki.

In: Biochimica et Biophysica Acta - Molecular Basis of Disease, Vol. 1812, No. 12, 12.2011, p. 1577-1583.

Research output: Contribution to journalArticle

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