Molecular signatures to define spermatogenic cells in common marmoset (Callithrix jacchus)

ZacharyYu Ching Lin, Masanori Imamura, Chiaki Sano, Ryusuke Nakajima, Tomoko Suzuki, Rie Yamadera, Yuji Takehara, Hirotaka James Okano, Erika Sasaki, Hideyuki Okano

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Germ cell development is a fundamental process required to produce offspring. The developmental program of spermatogenesis has been assumed to be similar among mammals. However, recent studies have revealed differences in the molecular properties of primate germ cells compared with the well-characterized mouse germ cells. This may prevent simple application of rodent insights into higher primates. Therefore, thorough investigation of primate germ cells is necessary, as this may lead to the development of more appropriate animal models. The aim of this study is to define molecular signatures of spermatogenic cells in the common marmoset, Callithrix jacchus. Interestingly, NANOG, PRDM1, DPPA3 (STELLA), IFITM3, and ZP1 transcripts, but no POU5F1 (OCT4), were detected in adult marmoset testis. Conversely, mouse testis expressed Pou5f1 but not Nanog, Prdm1, Dppa3, Ifitm3, and Zp1. Other previously described mouse germ cell markers were conserved in marmoset and mouse testes. Intriguingly, marmoset spermatogenic cells underwent dynamic protein expression in a developmental stage-specific manner; DDX4 (VASA) protein was present in gonocytes, diminished in spermatogonial cells, and reexpressed in spermatocytes. To investigate epigenetic differences between adult marmoset and mice, DNA methylation analyses identified unique epigenetic profiles to marmoset and mice. Marmoset NANOG and POU5F1 promoters in spermatogenic cells exhibited a methylation status opposite to that in mice, while the DDX4 and LEFTY1 loci, as well as imprinted genes, displayed an evolutionarily conserved methylation pattern. Marmosets have great advantages as models for human reproductive biology and are also valuable as experimental nonhuman primates; thus, the current study provides an important platform for primate reproductive biology, including possible applications to humans.

Original languageEnglish
Pages (from-to)597-609
Number of pages13
JournalReproduction
Volume143
Issue number5
DOIs
Publication statusPublished - 2012 May

Fingerprint

Callithrix
Germ Cells
Primates
Testis
Epigenomics
Methylation
Spermatocytes
DNA Methylation
Spermatogenesis
Mammals
Rodentia
Proteins
Animal Models

ASJC Scopus subject areas

  • Obstetrics and Gynaecology
  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Cell Biology

Cite this

Lin, Z. C., Imamura, M., Sano, C., Nakajima, R., Suzuki, T., Yamadera, R., ... Okano, H. (2012). Molecular signatures to define spermatogenic cells in common marmoset (Callithrix jacchus). Reproduction, 143(5), 597-609. https://doi.org/10.1530/REP-11-0215

Molecular signatures to define spermatogenic cells in common marmoset (Callithrix jacchus). / Lin, ZacharyYu Ching; Imamura, Masanori; Sano, Chiaki; Nakajima, Ryusuke; Suzuki, Tomoko; Yamadera, Rie; Takehara, Yuji; Okano, Hirotaka James; Sasaki, Erika; Okano, Hideyuki.

In: Reproduction, Vol. 143, No. 5, 05.2012, p. 597-609.

Research output: Contribution to journalArticle

Lin, ZC, Imamura, M, Sano, C, Nakajima, R, Suzuki, T, Yamadera, R, Takehara, Y, Okano, HJ, Sasaki, E & Okano, H 2012, 'Molecular signatures to define spermatogenic cells in common marmoset (Callithrix jacchus)', Reproduction, vol. 143, no. 5, pp. 597-609. https://doi.org/10.1530/REP-11-0215
Lin ZC, Imamura M, Sano C, Nakajima R, Suzuki T, Yamadera R et al. Molecular signatures to define spermatogenic cells in common marmoset (Callithrix jacchus). Reproduction. 2012 May;143(5):597-609. https://doi.org/10.1530/REP-11-0215
Lin, ZacharyYu Ching ; Imamura, Masanori ; Sano, Chiaki ; Nakajima, Ryusuke ; Suzuki, Tomoko ; Yamadera, Rie ; Takehara, Yuji ; Okano, Hirotaka James ; Sasaki, Erika ; Okano, Hideyuki. / Molecular signatures to define spermatogenic cells in common marmoset (Callithrix jacchus). In: Reproduction. 2012 ; Vol. 143, No. 5. pp. 597-609.
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