Molecular species analysis of glycosphingolipids from small intestine of Japanese quail, Coturnix coturnix Japonica by HPLC/FAB/MS

Atsushi C Suzuki, Akira Nakamura, Kenji Nishimura

Research output: Contribution to journalArticle

Abstract

Neutral glycosphingolipids were isolated from quail small intestine and their structures were analysed. They contained: Galβ1-4GlcCer(LacCer), Galα1-4GalCer(Ga2Cer), Galα1-4Galβ1-4GlcCer(Gb3Cer), GlcNAcβ1-3Galβ1-4GlcCer(Le3Cer), GalNAcβ1-4Galβ1-4GlcCer(Gg3Cer), GalNAcβ1-4[GalNAcβ1-3]Galβ1-4GlcCer(LcGg4Cer), and GalNAcα1-3GalNAcβ1-3Galα1-4Galβ1-4GlcCer (Forssman glycolipid) as well as glucosylceramide, galactosylceramide (Nishimura K et al. 1984)Biochim Biophys Acta796:269-76) and the Lex glycolipid, III3 Fucα-nLc4Cer (Nishimura K et al. (1989)J. Biochem (Tokyo)101:1315-18). The molecular species compositions of these glycosphingolipids were examined using fast atom bombardment-mass spectrometry linked with reversed-phase high-performance liquid chromatography. By such analysis, we could classify the quail glycosphingolipids into at least three classes: glycolipids rich in species having four hydroxyl groups in the ceramides (GalCer, Gg3Cer, LcGg4Cer and Lex), those rich in the ceramides of N-acyl trihydroxysphinganine with normal fatty acids (Lc3Cer), and glycolipids rich in the ceramides of N-acyl sphingenine with normal fatty acids (LacCer, Gb3Cer and Forssman glycolipid). Immunohistochemical observation implies that the differences in the hydrophobic moieties specified the localization of glycosphingolipids in the tissue.

Original languageEnglish
Pages (from-to)111-121
Number of pages11
JournalGlycoconjugate Journal
Volume11
Issue number2
DOIs
Publication statusPublished - 1994 Apr

Fingerprint

Coturnix
Glycosphingolipids
Ceramides
Glycolipids
Small Intestine
Quail
High Pressure Liquid Chromatography
Fatty Acids
Neutral Glycosphingolipids
Galactosylceramides
Glucosylceramides
Fast Atom Bombardment Mass Spectrometry
Tokyo
High performance liquid chromatography
Reverse-Phase Chromatography
Hydroxyl Radical
Mass spectrometry
Observation
Tissue
Atoms

Keywords

  • glycosphingolipids
  • HPLC/FAB/MS
  • immunohistochemistry
  • quail
  • small intestine

ASJC Scopus subject areas

  • Biochemistry

Cite this

Molecular species analysis of glycosphingolipids from small intestine of Japanese quail, Coturnix coturnix Japonica by HPLC/FAB/MS. / Suzuki, Atsushi C; Nakamura, Akira; Nishimura, Kenji.

In: Glycoconjugate Journal, Vol. 11, No. 2, 04.1994, p. 111-121.

Research output: Contribution to journalArticle

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abstract = "Neutral glycosphingolipids were isolated from quail small intestine and their structures were analysed. They contained: Galβ1-4GlcCer(LacCer), Galα1-4GalCer(Ga2Cer), Galα1-4Galβ1-4GlcCer(Gb3Cer), GlcNAcβ1-3Galβ1-4GlcCer(Le3Cer), GalNAcβ1-4Galβ1-4GlcCer(Gg3Cer), GalNAcβ1-4[GalNAcβ1-3]Galβ1-4GlcCer(LcGg4Cer), and GalNAcα1-3GalNAcβ1-3Galα1-4Galβ1-4GlcCer (Forssman glycolipid) as well as glucosylceramide, galactosylceramide (Nishimura K et al. 1984)Biochim Biophys Acta796:269-76) and the Lex glycolipid, III3 Fucα-nLc4Cer (Nishimura K et al. (1989)J. Biochem (Tokyo)101:1315-18). The molecular species compositions of these glycosphingolipids were examined using fast atom bombardment-mass spectrometry linked with reversed-phase high-performance liquid chromatography. By such analysis, we could classify the quail glycosphingolipids into at least three classes: glycolipids rich in species having four hydroxyl groups in the ceramides (GalCer, Gg3Cer, LcGg4Cer and Lex), those rich in the ceramides of N-acyl trihydroxysphinganine with normal fatty acids (Lc3Cer), and glycolipids rich in the ceramides of N-acyl sphingenine with normal fatty acids (LacCer, Gb3Cer and Forssman glycolipid). Immunohistochemical observation implies that the differences in the hydrophobic moieties specified the localization of glycosphingolipids in the tissue.",
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N2 - Neutral glycosphingolipids were isolated from quail small intestine and their structures were analysed. They contained: Galβ1-4GlcCer(LacCer), Galα1-4GalCer(Ga2Cer), Galα1-4Galβ1-4GlcCer(Gb3Cer), GlcNAcβ1-3Galβ1-4GlcCer(Le3Cer), GalNAcβ1-4Galβ1-4GlcCer(Gg3Cer), GalNAcβ1-4[GalNAcβ1-3]Galβ1-4GlcCer(LcGg4Cer), and GalNAcα1-3GalNAcβ1-3Galα1-4Galβ1-4GlcCer (Forssman glycolipid) as well as glucosylceramide, galactosylceramide (Nishimura K et al. 1984)Biochim Biophys Acta796:269-76) and the Lex glycolipid, III3 Fucα-nLc4Cer (Nishimura K et al. (1989)J. Biochem (Tokyo)101:1315-18). The molecular species compositions of these glycosphingolipids were examined using fast atom bombardment-mass spectrometry linked with reversed-phase high-performance liquid chromatography. By such analysis, we could classify the quail glycosphingolipids into at least three classes: glycolipids rich in species having four hydroxyl groups in the ceramides (GalCer, Gg3Cer, LcGg4Cer and Lex), those rich in the ceramides of N-acyl trihydroxysphinganine with normal fatty acids (Lc3Cer), and glycolipids rich in the ceramides of N-acyl sphingenine with normal fatty acids (LacCer, Gb3Cer and Forssman glycolipid). Immunohistochemical observation implies that the differences in the hydrophobic moieties specified the localization of glycosphingolipids in the tissue.

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