Moment analysis of drug disposition in kidney

Transcellular transport kinetics of p-aminohippurate in the isolated perfused rat kidney

R. Hori, Yusuke Tanigawara, Y. Saito, Y. Hayashi, T. Aiba, K. Okumura, A. Kamiya

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The mean renal epithelial cell residence time, T̄(cell), was defined as a model-independent characteristic of the transcellular transport process in the isolated perfused kidney. The transcellular transport process includes transport at the basolateral membrane, diffusion in the cytosol, and transport at the brush border membrane. The parameter T̄(cell) represents the mean time for the drug secreted from the tubules to pass through the renal epithelial cells, and is calculated as the difference of the mean urinary transit time between secreted drug and inulin in the single-pass perfusion system. Therefore, the urinary excretion rate-time course is indispensable to evaluate T̄(cell). p-Aminohippuric acid was used as a model compound. The bovine erythrocytes in the perfusate kept the isolated kidney in an almost constant physiological condition, including secretion function. The renal vein outflow curves were also analyzed by the use of moments. The dispersion in the cathether was corrected by a deconvolution. The apparent secretion intrinsic clearance and the apparent volume of distribution were calculated from the moments. The present method will be useful for analysis of the transcellular transport mechanism and the effect of disease states on renal transport of drugs.

Original languageEnglish
Pages (from-to)471-476
Number of pages6
JournalJournal of Pharmaceutical Sciences
Volume77
Issue number6
Publication statusPublished - 1988
Externally publishedYes

Fingerprint

p-Aminohippuric Acid
Transcytosis
T-cells
Rats
Kidney
Kinetics
Pharmaceutical Preparations
Membranes
T-Lymphocytes
Inulin
Deconvolution
Brushes
Epithelial Cells
Renal Veins
Microvilli
Cytosol
Perfusion
Erythrocytes

ASJC Scopus subject areas

  • Molecular Medicine
  • Chemistry(all)
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Pharmacology

Cite this

Moment analysis of drug disposition in kidney : Transcellular transport kinetics of p-aminohippurate in the isolated perfused rat kidney. / Hori, R.; Tanigawara, Yusuke; Saito, Y.; Hayashi, Y.; Aiba, T.; Okumura, K.; Kamiya, A.

In: Journal of Pharmaceutical Sciences, Vol. 77, No. 6, 1988, p. 471-476.

Research output: Contribution to journalArticle

@article{603ab5970bdf4bb29121df7a2d352abc,
title = "Moment analysis of drug disposition in kidney: Transcellular transport kinetics of p-aminohippurate in the isolated perfused rat kidney",
abstract = "The mean renal epithelial cell residence time, T̄(cell), was defined as a model-independent characteristic of the transcellular transport process in the isolated perfused kidney. The transcellular transport process includes transport at the basolateral membrane, diffusion in the cytosol, and transport at the brush border membrane. The parameter T̄(cell) represents the mean time for the drug secreted from the tubules to pass through the renal epithelial cells, and is calculated as the difference of the mean urinary transit time between secreted drug and inulin in the single-pass perfusion system. Therefore, the urinary excretion rate-time course is indispensable to evaluate T̄(cell). p-Aminohippuric acid was used as a model compound. The bovine erythrocytes in the perfusate kept the isolated kidney in an almost constant physiological condition, including secretion function. The renal vein outflow curves were also analyzed by the use of moments. The dispersion in the cathether was corrected by a deconvolution. The apparent secretion intrinsic clearance and the apparent volume of distribution were calculated from the moments. The present method will be useful for analysis of the transcellular transport mechanism and the effect of disease states on renal transport of drugs.",
author = "R. Hori and Yusuke Tanigawara and Y. Saito and Y. Hayashi and T. Aiba and K. Okumura and A. Kamiya",
year = "1988",
language = "English",
volume = "77",
pages = "471--476",
journal = "Journal of Pharmaceutical Sciences",
issn = "0022-3549",
publisher = "John Wiley and Sons Inc.",
number = "6",

}

TY - JOUR

T1 - Moment analysis of drug disposition in kidney

T2 - Transcellular transport kinetics of p-aminohippurate in the isolated perfused rat kidney

AU - Hori, R.

AU - Tanigawara, Yusuke

AU - Saito, Y.

AU - Hayashi, Y.

AU - Aiba, T.

AU - Okumura, K.

AU - Kamiya, A.

PY - 1988

Y1 - 1988

N2 - The mean renal epithelial cell residence time, T̄(cell), was defined as a model-independent characteristic of the transcellular transport process in the isolated perfused kidney. The transcellular transport process includes transport at the basolateral membrane, diffusion in the cytosol, and transport at the brush border membrane. The parameter T̄(cell) represents the mean time for the drug secreted from the tubules to pass through the renal epithelial cells, and is calculated as the difference of the mean urinary transit time between secreted drug and inulin in the single-pass perfusion system. Therefore, the urinary excretion rate-time course is indispensable to evaluate T̄(cell). p-Aminohippuric acid was used as a model compound. The bovine erythrocytes in the perfusate kept the isolated kidney in an almost constant physiological condition, including secretion function. The renal vein outflow curves were also analyzed by the use of moments. The dispersion in the cathether was corrected by a deconvolution. The apparent secretion intrinsic clearance and the apparent volume of distribution were calculated from the moments. The present method will be useful for analysis of the transcellular transport mechanism and the effect of disease states on renal transport of drugs.

AB - The mean renal epithelial cell residence time, T̄(cell), was defined as a model-independent characteristic of the transcellular transport process in the isolated perfused kidney. The transcellular transport process includes transport at the basolateral membrane, diffusion in the cytosol, and transport at the brush border membrane. The parameter T̄(cell) represents the mean time for the drug secreted from the tubules to pass through the renal epithelial cells, and is calculated as the difference of the mean urinary transit time between secreted drug and inulin in the single-pass perfusion system. Therefore, the urinary excretion rate-time course is indispensable to evaluate T̄(cell). p-Aminohippuric acid was used as a model compound. The bovine erythrocytes in the perfusate kept the isolated kidney in an almost constant physiological condition, including secretion function. The renal vein outflow curves were also analyzed by the use of moments. The dispersion in the cathether was corrected by a deconvolution. The apparent secretion intrinsic clearance and the apparent volume of distribution were calculated from the moments. The present method will be useful for analysis of the transcellular transport mechanism and the effect of disease states on renal transport of drugs.

UR - http://www.scopus.com/inward/record.url?scp=0024209426&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024209426&partnerID=8YFLogxK

M3 - Article

VL - 77

SP - 471

EP - 476

JO - Journal of Pharmaceutical Sciences

JF - Journal of Pharmaceutical Sciences

SN - 0022-3549

IS - 6

ER -