TY - JOUR
T1 - Monitoring the morphological evolution of giant vesicles by azo dye-based sum-frequency generation (SFG) microscopy
AU - Momotake, Atsuya
AU - Mizuguchi, Takaha
AU - Hishida, Mafumi
AU - Yamamoto, Yasuhiko
AU - Yasui, Masato
AU - Nuriya, Mutsuo
N1 - Funding Information:
This work was supported by JSPS KAKENHI ( 16H01434 and 18K05312 ) and JST PRESTO ( JPMJPR17G6 ). We would like to thank Olympus Corporation for continuous support in the development of multiphoton microscopy. We would like to thank Enago ( www.enago.jp ) for the English language review. Appendix A
Funding Information:
This work was supported by JSPS KAKENHI (16H01434 and 18K05312) and JST PRESTO (JPMJPR17G6). We would like to thank Olympus Corporation for continuous support in the development of multiphoton microscopy. We would like to thank Enago (www.enago.jp) for the English language review.
Publisher Copyright:
© 2019 Elsevier B.V.
PY - 2020/2
Y1 - 2020/2
N2 - In the present work, dye-based sum-frequency generation (SFG) imaging using sodium 4-[4-(dibutylamino)phenylazo]benzenesulfonate (butyl orange, BO) as a new non-fluorescent specific azo dye is employed to monitor the morphological evolution of giant vesicles (GVs). After loading BO to the membrane of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) single-component GVs, the outermost membranes were clearly visualized using SFG microscopy, which provided images of the distinct outer and inner faces of the lipid bilayers. In addition, SFG-active vesicles were detected also inside the GVs, depending on the dye concentrations. The dye-based SFG imaging technique provided experimental evidence that these oligolamellar vesicles containing an SFG-active interior had been formed after BO loading. The formation process of the oligolamellar vesicles with inner SFG-active vesicles was successfully monitored, and their formation mechanism was discussed.
AB - In the present work, dye-based sum-frequency generation (SFG) imaging using sodium 4-[4-(dibutylamino)phenylazo]benzenesulfonate (butyl orange, BO) as a new non-fluorescent specific azo dye is employed to monitor the morphological evolution of giant vesicles (GVs). After loading BO to the membrane of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) single-component GVs, the outermost membranes were clearly visualized using SFG microscopy, which provided images of the distinct outer and inner faces of the lipid bilayers. In addition, SFG-active vesicles were detected also inside the GVs, depending on the dye concentrations. The dye-based SFG imaging technique provided experimental evidence that these oligolamellar vesicles containing an SFG-active interior had been formed after BO loading. The formation process of the oligolamellar vesicles with inner SFG-active vesicles was successfully monitored, and their formation mechanism was discussed.
KW - Azo dye
KW - Giant vesicle
KW - Lipid bilayer
KW - Non-Fluorescent dye
KW - SFG
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U2 - 10.1016/j.colsurfb.2019.110716
DO - 10.1016/j.colsurfb.2019.110716
M3 - Article
C2 - 31865122
AN - SCOPUS:85076551650
VL - 186
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
M1 - 110716
ER -