Monoclonal antibodies distinctively recognizing the subtypes of inositol 1,4,5-trisphosphate receptor

Application to the studies on inflammatory cells

Tomoyasu Sugiyama, Akiko Furuya, Toshiaki Monkawa, Miki Yamamoto-Hino, Souichiro Satoh, Kenji Ohmori, Atsushi Miyawaki, Nobuo Hanai, Katsuhiko Mikoshiba, Mamoru Hasegawa

Research output: Contribution to journalArticle

80 Citations (Scopus)

Abstract

Monoclonal antibodies were raised that specifically recognize the COOH-terminal sequences and the loop sequences between the fifth and the sixth transmembrane spanning regions of human inositol 1,4,5-trisphosphate receptor (IP3R) type 1, 2 and 3. Western blot analysis using Jurkat cells, mouse cerebellum, COS-7 expressing IP3R type 3 cDNA showed that those monoclonal antibodies reacted specifically with each of these three IP3R subtypes and that they do not cross-react. These antibodies could be used for the specific immunoprecipitation of IP3Rs. Using these monoclonal antibodies, the expression profiles of IP3R-subtype proteins were found to be different among inflammatory cells such as macrophages, polymorphonuclear cells, mast cells, eosinophils, splenocytes, thymocytes and megakaryocytic cells. Usually, more than one type of IP3R were expressed in a cell simultaneously. The observation of CMK cells under immunofluorescence confocal microscopy revealed that IP3R type 1 and type 2 are located at different subcellular fractions.

Original languageEnglish
Pages (from-to)149-154
Number of pages6
JournalFEBS Letters
Volume354
Issue number2
DOIs
Publication statusPublished - 1994 Nov 7
Externally publishedYes

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Inositol 1,4,5-Trisphosphate Receptors
Monoclonal Antibodies
Confocal microscopy
Macrophages
Complementary DNA
Jurkat Cells
Subcellular Fractions
Thymocytes
Fluorescence Microscopy
Immunoprecipitation
Eosinophils
Mast Cells
Confocal Microscopy
Antibodies
Cerebellum
Western Blotting
Observation
Proteins

Keywords

  • Inositol 1,4,5-trisphosphate receptor
  • Intracellular calcium
  • Macrophage
  • Mast cell
  • Megakaryocyte
  • Monoclonal antibody
  • PMN
  • Splenocyte
  • Thymocyte

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Monoclonal antibodies distinctively recognizing the subtypes of inositol 1,4,5-trisphosphate receptor : Application to the studies on inflammatory cells. / Sugiyama, Tomoyasu; Furuya, Akiko; Monkawa, Toshiaki; Yamamoto-Hino, Miki; Satoh, Souichiro; Ohmori, Kenji; Miyawaki, Atsushi; Hanai, Nobuo; Mikoshiba, Katsuhiko; Hasegawa, Mamoru.

In: FEBS Letters, Vol. 354, No. 2, 07.11.1994, p. 149-154.

Research output: Contribution to journalArticle

Sugiyama, T, Furuya, A, Monkawa, T, Yamamoto-Hino, M, Satoh, S, Ohmori, K, Miyawaki, A, Hanai, N, Mikoshiba, K & Hasegawa, M 1994, 'Monoclonal antibodies distinctively recognizing the subtypes of inositol 1,4,5-trisphosphate receptor: Application to the studies on inflammatory cells', FEBS Letters, vol. 354, no. 2, pp. 149-154. https://doi.org/10.1016/0014-5793(94)01099-4
Sugiyama, Tomoyasu ; Furuya, Akiko ; Monkawa, Toshiaki ; Yamamoto-Hino, Miki ; Satoh, Souichiro ; Ohmori, Kenji ; Miyawaki, Atsushi ; Hanai, Nobuo ; Mikoshiba, Katsuhiko ; Hasegawa, Mamoru. / Monoclonal antibodies distinctively recognizing the subtypes of inositol 1,4,5-trisphosphate receptor : Application to the studies on inflammatory cells. In: FEBS Letters. 1994 ; Vol. 354, No. 2. pp. 149-154.
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