Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction

Yoshinori Miyahara, Noritoshi Nagaya, Masaharu Kataoka, Bobby Yanagawa, Koichi Tanaka, Hiroyuki Hao, Kozo Ishino, Hideyuki Ishida, Tatsuya Shimizu, Kenji Kangawa, Shunji Sano, Teruo Okano, Soichiro Kitamura, Hidezo Mori

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928 Citations (Scopus)

Abstract

Mesenchymal stem cells are multipotent cells that can differentiate into cardiomyocytes and vascular endothelial cells. Here we show, using cell sheet technology, that monolayered mesenchymal stem cells have multipotent and self-propagating properties after transplantation into infarcted rat hearts. We cultured adipose tissue-derived mesenchymal stem cells characterized by flow cytometry using temperature-responsive culture dishes. Four weeks after coronary ligation, we transplanted the monolayered mesenchymal stem cells onto the scarred myocardium. After transplantation, the engrafted sheet gradually grew to form a thick stratum that included newly formed vessels, undifferentiated cells and few cardiomyocytes. The mesenchymal stem cell sheet also acted through paracrine pathways to trigger angiogenesis. Unlike a fibroblast cell sheet, the monolayered mesenchymal stem cells reversed wall thinning in the scar area and improved cardiac function in rats with myocardial infarction. Thus, transplantation of monolayered mesenchymal stem cells may be a new therapeutic strategy for cardiac tissue regeneration.

Original languageEnglish
Pages (from-to)459-465
Number of pages7
JournalNature medicine
Volume12
Issue number4
DOIs
Publication statusPublished - 2006 Apr 1

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Miyahara, Y., Nagaya, N., Kataoka, M., Yanagawa, B., Tanaka, K., Hao, H., Ishino, K., Ishida, H., Shimizu, T., Kangawa, K., Sano, S., Okano, T., Kitamura, S., & Mori, H. (2006). Monolayered mesenchymal stem cells repair scarred myocardium after myocardial infarction. Nature medicine, 12(4), 459-465. https://doi.org/10.1038/nm1391