Monolithic silica rods grafted with thermoresponsive anionic polymer brushes for high-speed separation of basic biomolecules and peptides

Kenichi Nagase, Jun Kobayashi, Akihiko Kikuchi, Yoshikatsu Akiyama, Hideko Kanazawa, Teruo Okano

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Thermoresponsive anionic copolymer brushes, poly(N-isopropylacrylamide-co- acrylic acid-co-tert-butylacrylamide) [P(IPAAm-co-AAc-co-tBAAm)], were grafted onto a monolithic silica rod column through surface-initiated atom-transfer radical polymerization (ATRP) to prepare an effective thermoresponsive anionic chromatography matrix. An ATRP initiator was attached to the rod surface. N-Isopropylacrylamide (IPAAm), tert-butyl acrylate (tBA), tert-butylacrylamide (tBAAm), and the ATRP catalyst CuCl/CuCl<inf>2</inf>/tris[2-(N,N-dimethylamino) ethyl]amine were dissolved in 2-propanol, and the reaction mixture was pumped into the initiator-modified column. After grafting P(IPAAm-co-tBA-co-tBAAm) on the monolithic silica surfaces, deprotection of the tert-butyl group of tBA was performed. Chromatographic analysis showed that the prepared column was able to separate catecholamine derivatives and angiotensin subtypes within a shorter analysis time (5 min) than a silica-bead-packed column modified with the same copolymer brush could. These results indicated that the prepared copolymer-modified monolithic silica rod column may be a promising bioanalytical and bioseparation tool for rapid analysis of basic bioactive compounds and peptides.

Original languageEnglish
Pages (from-to)1204-1215
Number of pages12
JournalBiomacromolecules
Volume15
Issue number4
DOIs
Publication statusPublished - 2014 Apr 14

Fingerprint

Biomolecules
Brushes
Silicon Dioxide
Peptides
Atom transfer radical polymerization
Polymers
Silica
Polymerization
Copolymers
Chromatography
Chromatographic analysis
2-Propanol
Angiotensins
Propanol
Acrylics
Catecholamines
Amines
Derivatives
Catalysts
Acids

ASJC Scopus subject areas

  • Bioengineering
  • Materials Chemistry
  • Polymers and Plastics
  • Biomaterials
  • Medicine(all)

Cite this

Monolithic silica rods grafted with thermoresponsive anionic polymer brushes for high-speed separation of basic biomolecules and peptides. / Nagase, Kenichi; Kobayashi, Jun; Kikuchi, Akihiko; Akiyama, Yoshikatsu; Kanazawa, Hideko; Okano, Teruo.

In: Biomacromolecules, Vol. 15, No. 4, 14.04.2014, p. 1204-1215.

Research output: Contribution to journalArticle

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