Abstract
Vertical cell-cell contact is an important difference between 2D- and 3D-culture models. We hypothesized that this difference in cell-cell contacts is critical in maintaining hepatocyte functions. Here we developed a simple culture technique to investigate the effect of the vertical cell-cell contacts on morphogenesis, proliferation, and differentiation of hepatocytes. Two separate monolayers of primary rat hepatocytes were first cultured on a microporous membrane and a culture dish and one was then inverted and placed on top of the other to create a 3D-configuration. Imaging techniques revealed that hepatocytes recovered cell polarity and formed bile canaliculi in response to the addition of the vertical cell-cell contacts. Quantitative analysis revealed that the cells exhibited cuboidal cell shape with vertical cell-cell contacts and maintained differentiated functions while their proliferation activity was inhibited. In contrast, hepatocytes in a monolayer gradually flattened due to the lack of vertical cell-cell contacts, resulting in cells losing differentiated functions which correlated to the deformation of cell shape. Controlling 2D- and 3D-configurations is important in switching growth and differentiation of hepatocytes. These results implicate how we apply 2D- and 3D-cultures for tissue engineering of the liver.
| Original language | English |
|---|---|
| Pages (from-to) | 235-248 |
| Number of pages | 14 |
| Journal | Journal of Biomechanical Science and Engineering |
| Volume | 3 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2008 |
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Keywords
- 3D Culture
- Cell-Cell Adhesion
- Microporous Membrane
- Primary Hepatocyte
- Tissue Engineering
ASJC Scopus subject areas
- Biomedical Engineering
Cite this
Morphological and Functional Changes of Rat Hepatocytes by Vertical Cell-Cell Adhesion in Three-Dimensional Stacked-Up Culture. / Sudo, Ryo; Mitaka, Toshihiro; Ikeda, Mariko; Tanishita, Kazuo.
In: Journal of Biomechanical Science and Engineering, Vol. 3, No. 2, 2008, p. 235-248.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Morphological and Functional Changes of Rat Hepatocytes by Vertical Cell-Cell Adhesion in Three-Dimensional Stacked-Up Culture
AU - Sudo, Ryo
AU - Mitaka, Toshihiro
AU - Ikeda, Mariko
AU - Tanishita, Kazuo
PY - 2008
Y1 - 2008
N2 - Vertical cell-cell contact is an important difference between 2D- and 3D-culture models. We hypothesized that this difference in cell-cell contacts is critical in maintaining hepatocyte functions. Here we developed a simple culture technique to investigate the effect of the vertical cell-cell contacts on morphogenesis, proliferation, and differentiation of hepatocytes. Two separate monolayers of primary rat hepatocytes were first cultured on a microporous membrane and a culture dish and one was then inverted and placed on top of the other to create a 3D-configuration. Imaging techniques revealed that hepatocytes recovered cell polarity and formed bile canaliculi in response to the addition of the vertical cell-cell contacts. Quantitative analysis revealed that the cells exhibited cuboidal cell shape with vertical cell-cell contacts and maintained differentiated functions while their proliferation activity was inhibited. In contrast, hepatocytes in a monolayer gradually flattened due to the lack of vertical cell-cell contacts, resulting in cells losing differentiated functions which correlated to the deformation of cell shape. Controlling 2D- and 3D-configurations is important in switching growth and differentiation of hepatocytes. These results implicate how we apply 2D- and 3D-cultures for tissue engineering of the liver.
AB - Vertical cell-cell contact is an important difference between 2D- and 3D-culture models. We hypothesized that this difference in cell-cell contacts is critical in maintaining hepatocyte functions. Here we developed a simple culture technique to investigate the effect of the vertical cell-cell contacts on morphogenesis, proliferation, and differentiation of hepatocytes. Two separate monolayers of primary rat hepatocytes were first cultured on a microporous membrane and a culture dish and one was then inverted and placed on top of the other to create a 3D-configuration. Imaging techniques revealed that hepatocytes recovered cell polarity and formed bile canaliculi in response to the addition of the vertical cell-cell contacts. Quantitative analysis revealed that the cells exhibited cuboidal cell shape with vertical cell-cell contacts and maintained differentiated functions while their proliferation activity was inhibited. In contrast, hepatocytes in a monolayer gradually flattened due to the lack of vertical cell-cell contacts, resulting in cells losing differentiated functions which correlated to the deformation of cell shape. Controlling 2D- and 3D-configurations is important in switching growth and differentiation of hepatocytes. These results implicate how we apply 2D- and 3D-cultures for tissue engineering of the liver.
KW - 3D Culture
KW - Cell-Cell Adhesion
KW - Microporous Membrane
KW - Primary Hepatocyte
KW - Tissue Engineering
UR - http://www.scopus.com/inward/record.url?scp=85014165356&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85014165356&partnerID=8YFLogxK
U2 - 10.1299/jbse.3.235
DO - 10.1299/jbse.3.235
M3 - Article
AN - SCOPUS:85014165356
VL - 3
SP - 235
EP - 248
JO - Journal of Biomechanical Science and Engineering
JF - Journal of Biomechanical Science and Engineering
SN - 1880-9863
IS - 2
ER -