Morphological Control of Endothelial Tube Formation by Three-Dimensional Lane-Shaped Patterns

Yuki Ikezawa, Hiroaki Onoe

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper describes an analytical platform to control endothelial tube formation by patterning cells and extracellular matrix (ECM) within 3D lane-shaped pattern. By using PDMS groove patterns (50-250\ \mu \mathrm{m} in width and height, several millimeters in length), human umbilical vein endothelial cells (HUVECs) were patterned three-dimensionally in collagen gel. The patterned HUVECs spontaneously form endothelial vascular tissues, but their morphologies can be controlled by the dimensions of patterns, cell densities, ECMs, etc. We succeeded in forming vascular tube tissues, and found that a number of branches of the vascular tissues depends on the dimensions of 3D-patterns. We believe that our culture system could be a useful tool for understanding the tube formation behavior of endothelial cells and for creating 3D-shape-controlled vascular tissues as a quantitative model in vitro.

Original languageEnglish
Title of host publication2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages594-596
Number of pages3
ISBN (Electronic)9781728116105
DOIs
Publication statusPublished - 2019 Jan
Event32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019 - Seoul, Korea, Republic of
Duration: 2019 Jan 272019 Jan 31

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
Volume2019-January
ISSN (Print)1084-6999

Conference

Conference32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019
CountryKorea, Republic of
CitySeoul
Period19/1/2719/1/31

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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  • Cite this

    Ikezawa, Y., & Onoe, H. (2019). Morphological Control of Endothelial Tube Formation by Three-Dimensional Lane-Shaped Patterns. In 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019 (pp. 594-596). [8870622] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS); Vol. 2019-January). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/MEMSYS.2019.8870622