TY - GEN
T1 - Morphological Control of Endothelial Tube Formation by Three-Dimensional Lane-Shaped Patterns
AU - Ikezawa, Yuki
AU - Onoe, Hiroaki
N1 - Funding Information:
This work was partly supported by Grant-in-Aid for Challenging Exploratory Research (No. 26289060) from Japan Society of the Promotion of Science (JSPS), Japan.
Publisher Copyright:
© 2019 IEEE.
PY - 2019/1
Y1 - 2019/1
N2 - This paper describes an analytical platform to control endothelial tube formation by patterning cells and extracellular matrix (ECM) within 3D lane-shaped pattern. By using PDMS groove patterns (50-250\ \mu \mathrm{m} in width and height, several millimeters in length), human umbilical vein endothelial cells (HUVECs) were patterned three-dimensionally in collagen gel. The patterned HUVECs spontaneously form endothelial vascular tissues, but their morphologies can be controlled by the dimensions of patterns, cell densities, ECMs, etc. We succeeded in forming vascular tube tissues, and found that a number of branches of the vascular tissues depends on the dimensions of 3D-patterns. We believe that our culture system could be a useful tool for understanding the tube formation behavior of endothelial cells and for creating 3D-shape-controlled vascular tissues as a quantitative model in vitro.
AB - This paper describes an analytical platform to control endothelial tube formation by patterning cells and extracellular matrix (ECM) within 3D lane-shaped pattern. By using PDMS groove patterns (50-250\ \mu \mathrm{m} in width and height, several millimeters in length), human umbilical vein endothelial cells (HUVECs) were patterned three-dimensionally in collagen gel. The patterned HUVECs spontaneously form endothelial vascular tissues, but their morphologies can be controlled by the dimensions of patterns, cell densities, ECMs, etc. We succeeded in forming vascular tube tissues, and found that a number of branches of the vascular tissues depends on the dimensions of 3D-patterns. We believe that our culture system could be a useful tool for understanding the tube formation behavior of endothelial cells and for creating 3D-shape-controlled vascular tissues as a quantitative model in vitro.
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U2 - 10.1109/MEMSYS.2019.8870622
DO - 10.1109/MEMSYS.2019.8870622
M3 - Conference contribution
AN - SCOPUS:85074321062
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 594
EP - 596
BT - 2019 IEEE 32nd International Conference on Micro Electro Mechanical Systems, MEMS 2019
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 32nd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2019
Y2 - 27 January 2019 through 31 January 2019
ER -