Morphology and barrier function of the corneal epithelium after penetrating keratoplasty: Association with original diseases, tear function, and suture removal

Jun Shimazaki, Shigeto Shimmura, Kaori Mochizuki, Kazuo Tsubota

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Purpose. To study changes in structure and barrier function of the corneal epithelium after penetrating keratoplasty. Methods. Sixty-nine eyes of 56 patients undergoing penetrating keratoplasty were studied. Fifty-six age-matched normal eyes served as controls. Corneal epithelial structure was studied by using specular microscopy. Barrier function of the corneal epithelium was examined by slit-lamp fluorophotometry. Vital staining, tear function, and corneal sensitivity also were examined. Results. Fluorescein and rose bengal staining scores, incidence of elongated cells, the mean epithelial area, and mean fluorescein uptake were significantly increased in postkeratoplasty eyes compared with controls. Original disease influenced the results of barrier function, where keratoconus revealed better function than bullous keratopathy or corneal scar. However, no such differences were noted in epithelial structure. Six eyes with absence of palisades of Vogt had revealed significantly increased epithelial area and fluorescein uptake. Removal of the running suture caused a decrease in elongated cells and epithelial cell area without significant changes in barrier function. Conclusion. Corneal epithelial structure was significantly impaired in postkeratoplasty eyes. Epithelial barrier function of postkeratoplasty eyes was influenced by original diseases. Removal of sutures improves epithelial structure but not barrier function.

Original languageEnglish
Pages (from-to)559-564
Number of pages6
JournalCornea
Volume18
Issue number5
Publication statusPublished - 1999 Sep
Externally publishedYes

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Penetrating Keratoplasty
Corneal Epithelium
Tears
Sutures
Fluorescein
Epithelial Cells
Fluorophotometry
Staining and Labeling
Rose Bengal
Keratoconus
Microscopy
Incidence

Keywords

  • Barrier function
  • Corneal epithelium
  • Corneal transplantation
  • Fluorometry
  • Specular microscopy

ASJC Scopus subject areas

  • Ophthalmology

Cite this

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abstract = "Purpose. To study changes in structure and barrier function of the corneal epithelium after penetrating keratoplasty. Methods. Sixty-nine eyes of 56 patients undergoing penetrating keratoplasty were studied. Fifty-six age-matched normal eyes served as controls. Corneal epithelial structure was studied by using specular microscopy. Barrier function of the corneal epithelium was examined by slit-lamp fluorophotometry. Vital staining, tear function, and corneal sensitivity also were examined. Results. Fluorescein and rose bengal staining scores, incidence of elongated cells, the mean epithelial area, and mean fluorescein uptake were significantly increased in postkeratoplasty eyes compared with controls. Original disease influenced the results of barrier function, where keratoconus revealed better function than bullous keratopathy or corneal scar. However, no such differences were noted in epithelial structure. Six eyes with absence of palisades of Vogt had revealed significantly increased epithelial area and fluorescein uptake. Removal of the running suture caused a decrease in elongated cells and epithelial cell area without significant changes in barrier function. Conclusion. Corneal epithelial structure was significantly impaired in postkeratoplasty eyes. Epithelial barrier function of postkeratoplasty eyes was influenced by original diseases. Removal of sutures improves epithelial structure but not barrier function.",
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T1 - Morphology and barrier function of the corneal epithelium after penetrating keratoplasty

T2 - Association with original diseases, tear function, and suture removal

AU - Shimazaki, Jun

AU - Shimmura, Shigeto

AU - Mochizuki, Kaori

AU - Tsubota, Kazuo

PY - 1999/9

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N2 - Purpose. To study changes in structure and barrier function of the corneal epithelium after penetrating keratoplasty. Methods. Sixty-nine eyes of 56 patients undergoing penetrating keratoplasty were studied. Fifty-six age-matched normal eyes served as controls. Corneal epithelial structure was studied by using specular microscopy. Barrier function of the corneal epithelium was examined by slit-lamp fluorophotometry. Vital staining, tear function, and corneal sensitivity also were examined. Results. Fluorescein and rose bengal staining scores, incidence of elongated cells, the mean epithelial area, and mean fluorescein uptake were significantly increased in postkeratoplasty eyes compared with controls. Original disease influenced the results of barrier function, where keratoconus revealed better function than bullous keratopathy or corneal scar. However, no such differences were noted in epithelial structure. Six eyes with absence of palisades of Vogt had revealed significantly increased epithelial area and fluorescein uptake. Removal of the running suture caused a decrease in elongated cells and epithelial cell area without significant changes in barrier function. Conclusion. Corneal epithelial structure was significantly impaired in postkeratoplasty eyes. Epithelial barrier function of postkeratoplasty eyes was influenced by original diseases. Removal of sutures improves epithelial structure but not barrier function.

AB - Purpose. To study changes in structure and barrier function of the corneal epithelium after penetrating keratoplasty. Methods. Sixty-nine eyes of 56 patients undergoing penetrating keratoplasty were studied. Fifty-six age-matched normal eyes served as controls. Corneal epithelial structure was studied by using specular microscopy. Barrier function of the corneal epithelium was examined by slit-lamp fluorophotometry. Vital staining, tear function, and corneal sensitivity also were examined. Results. Fluorescein and rose bengal staining scores, incidence of elongated cells, the mean epithelial area, and mean fluorescein uptake were significantly increased in postkeratoplasty eyes compared with controls. Original disease influenced the results of barrier function, where keratoconus revealed better function than bullous keratopathy or corneal scar. However, no such differences were noted in epithelial structure. Six eyes with absence of palisades of Vogt had revealed significantly increased epithelial area and fluorescein uptake. Removal of the running suture caused a decrease in elongated cells and epithelial cell area without significant changes in barrier function. Conclusion. Corneal epithelial structure was significantly impaired in postkeratoplasty eyes. Epithelial barrier function of postkeratoplasty eyes was influenced by original diseases. Removal of sutures improves epithelial structure but not barrier function.

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KW - Corneal epithelium

KW - Corneal transplantation

KW - Fluorometry

KW - Specular microscopy

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