Mouse B-type lamins are required for proper organogenesis but not by embryonic stem cells

Youngjo Kim, Alexei A. Sharov, Katie McDole, Melody Cheng, Haiping Hao, Chen Ming Fan, Nicholas Gaiano, Minoru Ko, Yixian Zheng

Research output: Contribution to journalArticle

146 Citations (Scopus)

Abstract

B-type lamins, the major components of the nuclear lamina, are believed to be essential for cell proliferation and survival. We found that mouse embryonic stem cells (ESCs) do not need any lamins for self-renewal and pluripotency. Although genome-wide lamin-B binding profiles correlate with reduced gene expression, such binding is not directly required for gene silencing in ESCs or trophectoderm cells. However, B-type lamins are required for proper organogenesis. Defects in spindle orientation in neural progenitor cells and migration of neurons probably cause brain disorganizations found in lamin-B null mice. Thus, our studies not only disprove several prevailing views of lamin-Bs but also establish a foundation for redefining the function of the nuclear lamina in the context of tissue building and homeostasis.

Original languageEnglish
Pages (from-to)1706-1710
Number of pages5
JournalScience
Volume334
Issue number6063
DOIs
Publication statusPublished - 2011 Dec 23
Externally publishedYes

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Lamin Type B
Organogenesis
Embryonic Stem Cells
Nuclear Lamina
Lamins
Gene Silencing
Cell Movement
Cell Survival
Homeostasis
Stem Cells
Cell Proliferation
Genome
Gene Expression
Neurons
Brain

ASJC Scopus subject areas

  • General

Cite this

Kim, Y., Sharov, A. A., McDole, K., Cheng, M., Hao, H., Fan, C. M., ... Zheng, Y. (2011). Mouse B-type lamins are required for proper organogenesis but not by embryonic stem cells. Science, 334(6063), 1706-1710. https://doi.org/10.1126/science.1211222

Mouse B-type lamins are required for proper organogenesis but not by embryonic stem cells. / Kim, Youngjo; Sharov, Alexei A.; McDole, Katie; Cheng, Melody; Hao, Haiping; Fan, Chen Ming; Gaiano, Nicholas; Ko, Minoru; Zheng, Yixian.

In: Science, Vol. 334, No. 6063, 23.12.2011, p. 1706-1710.

Research output: Contribution to journalArticle

Kim, Y, Sharov, AA, McDole, K, Cheng, M, Hao, H, Fan, CM, Gaiano, N, Ko, M & Zheng, Y 2011, 'Mouse B-type lamins are required for proper organogenesis but not by embryonic stem cells', Science, vol. 334, no. 6063, pp. 1706-1710. https://doi.org/10.1126/science.1211222
Kim Y, Sharov AA, McDole K, Cheng M, Hao H, Fan CM et al. Mouse B-type lamins are required for proper organogenesis but not by embryonic stem cells. Science. 2011 Dec 23;334(6063):1706-1710. https://doi.org/10.1126/science.1211222
Kim, Youngjo ; Sharov, Alexei A. ; McDole, Katie ; Cheng, Melody ; Hao, Haiping ; Fan, Chen Ming ; Gaiano, Nicholas ; Ko, Minoru ; Zheng, Yixian. / Mouse B-type lamins are required for proper organogenesis but not by embryonic stem cells. In: Science. 2011 ; Vol. 334, No. 6063. pp. 1706-1710.
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