Mouse model of liver ischemia and reperfusion injury

method for studying reactive oxygen and nitrogen metabolites in vivo

Yuta Abe, Ian N. Hines, Gazi Zibari, Kevin Pavlick, Laura Gray, Yuukou Kitagawa, Matthew B. Grisham

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

The mouse model of liver ischemia and reperfusion injury has proven to be valuable for our understanding of the role that reactive oxygen and nitrogen metabolites play in postischemic tissue injury. This methods paper provides a detailed protocol for inducing partial liver ischemia followed by reperfusion. Liver ischemia is induced in anesthetized mice by cross-clamping the hepatic artery and portal vein for varying lengths of time, resulting in deprivation of blood flow to approximately 70% of the liver. Restoration of blood flow to the ischemic lobes enhances superoxide production concomitant with a rapid and marked decrease in the bioavailability of nitric oxide, resulting in alterations in the redox state of the liver in favor of a more oxidative environment. This hepatocellular oxidative stress induces the activation of oxidant-sensitive transcription factors followed by the upregulation of proinflammatory cytokines and mediators that ultimately lead to liver injury. This model can be induced in any strain or sex of mouse and requires 1-2 months of practice to become proficient in the surgery and animal manipulation. The roles of various reactive metabolites of oxygen and nitrogen may be evaluated using genetically engineered mice as well as selective molecular, cellular, and/or pharmacological agents.

Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalFree Radical Biology and Medicine
Volume46
Issue number1
DOIs
Publication statusPublished - 2009 Jan 1

Fingerprint

Metabolites
Reperfusion Injury
Liver
Nitrogen
Oxygen
Blood
Ischemia
Oxidative stress
Hepatic Veins
Hepatic Artery
Wounds and Injuries
Portal Vein
Oxidants
Constriction
Superoxides
Surgery
Biological Availability
Restoration
Reperfusion
Oxidation-Reduction

Keywords

  • Cytokines
  • Free radicals
  • Neutrophils
  • NF-κB

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Mouse model of liver ischemia and reperfusion injury : method for studying reactive oxygen and nitrogen metabolites in vivo. / Abe, Yuta; Hines, Ian N.; Zibari, Gazi; Pavlick, Kevin; Gray, Laura; Kitagawa, Yuukou; Grisham, Matthew B.

In: Free Radical Biology and Medicine, Vol. 46, No. 1, 01.01.2009, p. 1-7.

Research output: Contribution to journalArticle

Abe, Yuta ; Hines, Ian N. ; Zibari, Gazi ; Pavlick, Kevin ; Gray, Laura ; Kitagawa, Yuukou ; Grisham, Matthew B. / Mouse model of liver ischemia and reperfusion injury : method for studying reactive oxygen and nitrogen metabolites in vivo. In: Free Radical Biology and Medicine. 2009 ; Vol. 46, No. 1. pp. 1-7.
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