Mouse Models for Assessing the Protective Efficacy of Lactobacillus gasseri SBT2055 against Helicobacter suis Infection Associated with the Development of Gastric Mucosa-Associated Lymphoid Tissue Lymphoma

Hidenori Matsui, Tetsufumi Takahashi, Anders Øverby, Somay Yamagata Murayama, Haruno Yoshida, Yuji Yamamoto, Keita Nishiyama, Yasuyuki Seto, Takashi Takahashi, Takao Mukai, Masahiko Nakamura

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Helicobacter suis strain TKY infection has been strongly associated with the development of gastric mucosa-associated lymphoid tissue (MALT) lymphoma in a C57BL/6J mouse model. Materials and Methods: 1. C57BL/6J mice were intragastrically administered Lactobacillus strains once daily with 108-109 colony-forming units (CFU), starting 2 days before intragastric infection with H. suis TKY (approximately 1 × 104 copies of 16S rRNA genes) or H. pylori Sydney strain 1 (SS1; 3 × 108 CFU) and continuing for 14 days after infection. 2. C57BL/6J mice were given powdered feed mixed with lyophilized L. gasseri SBT2055 (LG2055) cells (5 × 108 CFU/g), starting 2 weeks before intragastric infection with H. suis TKY and continuing 12 months after infection. Results: 1. Among the 5 Lactobacillus strains that we examined, only LG2055 exhibited significantly preventive efficacy against both H. suis TKY and H. pylori SS1 at day 15 after infection. 2. Dietary supplementation with LG2055 protected mice from the formation of round protrusive lesions in the gastric fundus 12 months after infection with H. suis TKY, whereas such lesions had developed in the gastric fundus of nonsupplemented mice 12 months after infection. In addition, the formation of lymphoid follicles in gastric mucus layers was suppressed by dietary LG2055 at 3 months after infection. Conclusions: LG2055 administration is effective for suppressing the progression of gastric MALT lymphoma by reducing H. suis colonization.

Original languageEnglish
Pages (from-to)291-298
Number of pages8
JournalHelicobacter
Volume20
Issue number4
DOIs
Publication statusPublished - 2015 Aug 1
Externally publishedYes

Fingerprint

Helicobacter heilmannii
Marginal Zone B-Cell Lymphoma
Helicobacter Infections
Gastric Mucosa
Infection
Inbred C57BL Mouse
Gastric Fundus
Stem Cells
Pylorus
Lactobacillus
Lactobacillus gasseri
Mucus
Dietary Supplements
rRNA Genes
Stomach

Keywords

  • C57BL/6 mouse
  • Helicobacter suis
  • Lactobacillus gasseri
  • MALT lymphoma
  • Quantitative PCR

ASJC Scopus subject areas

  • Gastroenterology
  • Infectious Diseases

Cite this

Mouse Models for Assessing the Protective Efficacy of Lactobacillus gasseri SBT2055 against Helicobacter suis Infection Associated with the Development of Gastric Mucosa-Associated Lymphoid Tissue Lymphoma. / Matsui, Hidenori; Takahashi, Tetsufumi; Øverby, Anders; Murayama, Somay Yamagata; Yoshida, Haruno; Yamamoto, Yuji; Nishiyama, Keita; Seto, Yasuyuki; Takahashi, Takashi; Mukai, Takao; Nakamura, Masahiko.

In: Helicobacter, Vol. 20, No. 4, 01.08.2015, p. 291-298.

Research output: Contribution to journalArticle

Matsui, Hidenori ; Takahashi, Tetsufumi ; Øverby, Anders ; Murayama, Somay Yamagata ; Yoshida, Haruno ; Yamamoto, Yuji ; Nishiyama, Keita ; Seto, Yasuyuki ; Takahashi, Takashi ; Mukai, Takao ; Nakamura, Masahiko. / Mouse Models for Assessing the Protective Efficacy of Lactobacillus gasseri SBT2055 against Helicobacter suis Infection Associated with the Development of Gastric Mucosa-Associated Lymphoid Tissue Lymphoma. In: Helicobacter. 2015 ; Vol. 20, No. 4. pp. 291-298.
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abstract = "Background: Helicobacter suis strain TKY infection has been strongly associated with the development of gastric mucosa-associated lymphoid tissue (MALT) lymphoma in a C57BL/6J mouse model. Materials and Methods: 1. C57BL/6J mice were intragastrically administered Lactobacillus strains once daily with 108-109 colony-forming units (CFU), starting 2 days before intragastric infection with H. suis TKY (approximately 1 × 104 copies of 16S rRNA genes) or H. pylori Sydney strain 1 (SS1; 3 × 108 CFU) and continuing for 14 days after infection. 2. C57BL/6J mice were given powdered feed mixed with lyophilized L. gasseri SBT2055 (LG2055) cells (5 × 108 CFU/g), starting 2 weeks before intragastric infection with H. suis TKY and continuing 12 months after infection. Results: 1. Among the 5 Lactobacillus strains that we examined, only LG2055 exhibited significantly preventive efficacy against both H. suis TKY and H. pylori SS1 at day 15 after infection. 2. Dietary supplementation with LG2055 protected mice from the formation of round protrusive lesions in the gastric fundus 12 months after infection with H. suis TKY, whereas such lesions had developed in the gastric fundus of nonsupplemented mice 12 months after infection. In addition, the formation of lymphoid follicles in gastric mucus layers was suppressed by dietary LG2055 at 3 months after infection. Conclusions: LG2055 administration is effective for suppressing the progression of gastric MALT lymphoma by reducing H. suis colonization.",
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T1 - Mouse Models for Assessing the Protective Efficacy of Lactobacillus gasseri SBT2055 against Helicobacter suis Infection Associated with the Development of Gastric Mucosa-Associated Lymphoid Tissue Lymphoma

AU - Matsui, Hidenori

AU - Takahashi, Tetsufumi

AU - Øverby, Anders

AU - Murayama, Somay Yamagata

AU - Yoshida, Haruno

AU - Yamamoto, Yuji

AU - Nishiyama, Keita

AU - Seto, Yasuyuki

AU - Takahashi, Takashi

AU - Mukai, Takao

AU - Nakamura, Masahiko

PY - 2015/8/1

Y1 - 2015/8/1

N2 - Background: Helicobacter suis strain TKY infection has been strongly associated with the development of gastric mucosa-associated lymphoid tissue (MALT) lymphoma in a C57BL/6J mouse model. Materials and Methods: 1. C57BL/6J mice were intragastrically administered Lactobacillus strains once daily with 108-109 colony-forming units (CFU), starting 2 days before intragastric infection with H. suis TKY (approximately 1 × 104 copies of 16S rRNA genes) or H. pylori Sydney strain 1 (SS1; 3 × 108 CFU) and continuing for 14 days after infection. 2. C57BL/6J mice were given powdered feed mixed with lyophilized L. gasseri SBT2055 (LG2055) cells (5 × 108 CFU/g), starting 2 weeks before intragastric infection with H. suis TKY and continuing 12 months after infection. Results: 1. Among the 5 Lactobacillus strains that we examined, only LG2055 exhibited significantly preventive efficacy against both H. suis TKY and H. pylori SS1 at day 15 after infection. 2. Dietary supplementation with LG2055 protected mice from the formation of round protrusive lesions in the gastric fundus 12 months after infection with H. suis TKY, whereas such lesions had developed in the gastric fundus of nonsupplemented mice 12 months after infection. In addition, the formation of lymphoid follicles in gastric mucus layers was suppressed by dietary LG2055 at 3 months after infection. Conclusions: LG2055 administration is effective for suppressing the progression of gastric MALT lymphoma by reducing H. suis colonization.

AB - Background: Helicobacter suis strain TKY infection has been strongly associated with the development of gastric mucosa-associated lymphoid tissue (MALT) lymphoma in a C57BL/6J mouse model. Materials and Methods: 1. C57BL/6J mice were intragastrically administered Lactobacillus strains once daily with 108-109 colony-forming units (CFU), starting 2 days before intragastric infection with H. suis TKY (approximately 1 × 104 copies of 16S rRNA genes) or H. pylori Sydney strain 1 (SS1; 3 × 108 CFU) and continuing for 14 days after infection. 2. C57BL/6J mice were given powdered feed mixed with lyophilized L. gasseri SBT2055 (LG2055) cells (5 × 108 CFU/g), starting 2 weeks before intragastric infection with H. suis TKY and continuing 12 months after infection. Results: 1. Among the 5 Lactobacillus strains that we examined, only LG2055 exhibited significantly preventive efficacy against both H. suis TKY and H. pylori SS1 at day 15 after infection. 2. Dietary supplementation with LG2055 protected mice from the formation of round protrusive lesions in the gastric fundus 12 months after infection with H. suis TKY, whereas such lesions had developed in the gastric fundus of nonsupplemented mice 12 months after infection. In addition, the formation of lymphoid follicles in gastric mucus layers was suppressed by dietary LG2055 at 3 months after infection. Conclusions: LG2055 administration is effective for suppressing the progression of gastric MALT lymphoma by reducing H. suis colonization.

KW - C57BL/6 mouse

KW - Helicobacter suis

KW - Lactobacillus gasseri

KW - MALT lymphoma

KW - Quantitative PCR

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