M(r) 85,000 membrane protein specifically expressed in adriamycin-resistant human tumor cells

H. Hamada, E. Okochi, M. Watanabe, T. Oh-hara, Yoshikazu Sugimoto, H. Kawabata, T. Tsuruo

Research output: Contribution to journalArticle

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Abstract

For the characterization of membrane changes related to Adriamycin resistance in tumor cells, we have developed monoclonal antibodies against Adriamycin-resistant human myelogenous leukemia K562 (K562/ADM). In addition to the monoclonal antibodies which recognize P-glycoprotein, we have obtained two monoclonal antibodies (designated MRK4 and MRK20) which recognize an M(r) 85,000 membrane protein. Using MRK20 as a probe, we have studied the expression of the M(r) 85,000 protein in various human multidrug-resistant and -sensitive cell lines. The M(r) 85,000 protein was overexpressed in K562/ADM and in a human ovarian cancer cell line resistant to Adriamycin, 2780(AD). The protein, if any, was not detected in other drug-resistant human cell lines such as colchicine-resistant KB cells (KB-C4), vinblastine-resistant CEM cells (CEM/VLB100), and vincristine-resistant K562 cells (K562/VCR). We have isolated subclones of K562/ADM cells which express different amounts of the M(r) 85,000 protein. The expression of the M(r) 85,000 protein diminished when the cells were not kept in Adriamycin, and increased when the clones were kept in the presence of Adriamycin. In contrast, the expression of P-glycoprotein remained constant whether in the presence or absence of Adriamycin during these experiments. These findings suggest that the M(r) 85,000 membrane protein is closely related to the resistant mechanism specific to Adriamycin resistance, which is different from that of the pleiotropic drug resistance.

Original languageEnglish
Pages (from-to)7082-7087
Number of pages6
JournalCancer Research
Volume48
Issue number24 I
Publication statusPublished - 1988
Externally publishedYes

Fingerprint

Doxorubicin
Membrane Proteins
Neoplasms
K562 Cells
Monoclonal Antibodies
P-Glycoprotein
Proteins
Cell Line
KB Cells
Myeloid Leukemia
Vinblastine
Colchicine
Vincristine
Drug Resistance
Ovarian Neoplasms
Clone Cells
Membranes
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Hamada, H., Okochi, E., Watanabe, M., Oh-hara, T., Sugimoto, Y., Kawabata, H., & Tsuruo, T. (1988). M(r) 85,000 membrane protein specifically expressed in adriamycin-resistant human tumor cells. Cancer Research, 48(24 I), 7082-7087.

M(r) 85,000 membrane protein specifically expressed in adriamycin-resistant human tumor cells. / Hamada, H.; Okochi, E.; Watanabe, M.; Oh-hara, T.; Sugimoto, Yoshikazu; Kawabata, H.; Tsuruo, T.

In: Cancer Research, Vol. 48, No. 24 I, 1988, p. 7082-7087.

Research output: Contribution to journalArticle

Hamada, H, Okochi, E, Watanabe, M, Oh-hara, T, Sugimoto, Y, Kawabata, H & Tsuruo, T 1988, 'M(r) 85,000 membrane protein specifically expressed in adriamycin-resistant human tumor cells', Cancer Research, vol. 48, no. 24 I, pp. 7082-7087.
Hamada H, Okochi E, Watanabe M, Oh-hara T, Sugimoto Y, Kawabata H et al. M(r) 85,000 membrane protein specifically expressed in adriamycin-resistant human tumor cells. Cancer Research. 1988;48(24 I):7082-7087.
Hamada, H. ; Okochi, E. ; Watanabe, M. ; Oh-hara, T. ; Sugimoto, Yoshikazu ; Kawabata, H. ; Tsuruo, T. / M(r) 85,000 membrane protein specifically expressed in adriamycin-resistant human tumor cells. In: Cancer Research. 1988 ; Vol. 48, No. 24 I. pp. 7082-7087.
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