MRI Characterization of Paranodal Junction Failure and Related Spinal Cord Changes in Mice

Morito Takano, Keigo Hikishima, Kanehiro Fujiyoshi, Shinsuke Shibata, Akimasa Yasuda, Tsunehiko Konomi, Akiko Hayashi, Hiroko Baba, Koichi Honke, Yoshiaki Toyama, Hideyuki Okano, Masaya Nakamura

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The paranodal junction is a specialized axon-glia contact zone that is important for normal neuronal activity and behavioral locomotor function in the central nervous system (CNS). Histological examination has been the only method for detecting pathological paranodal junction conditions. Recently, diffusion tensor MRI (DTI) has been used to detect microstructural changes in various CNS diseases. This study was conducted to determine whether MRI and DTI could detect structural changes in the paranodal junctions of the spinal cord in cerebroside sulfotransferase knock-out (CST-KO) mice. Here, we showed that high-resolution MRI and DTI characteristics can reflect paranodal junction failure in CST-KO mice. We found significantly lower T1 times and significantly higher T2 times in the spinal cord MRIs of CST-KO mice as compared to wild-type (WT) mice. Spinal cord DTI showed significantly lower axial diffusivity and significantly higher radial diffusivity in CST-KO mice as compared to WT mice. In contrast, the histological differences in the paranodal junctions of WT and CST-KO mice were so subtle that electron microscopy or immunohistological analyses were necessary to detect them. We also measured gait disturbance in the CST-KO mice, and determined the conduction latency by electrophysiology. These findings demonstrate the potential of using MRI and DTI to evaluate white matter disorders that involve paranodal junction failure.

Original languageEnglish
Article numbere52904
JournalPLoS One
Volume7
Issue number12
DOIs
Publication statusPublished - 2012 Dec 27

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galactosylceramide sulfotransferase
cerebrosides
sulfotransferases
Diffusion Magnetic Resonance Imaging
Knockout Mice
spinal cord
Magnetic resonance imaging
Spinal Cord
mice
Tensors
Neurology
diffusivity
Electrophysiology
Central Nervous System Diseases
Locomotion
Gait
Neuroglia
central nervous system diseases
electrophysiology
Axons

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

MRI Characterization of Paranodal Junction Failure and Related Spinal Cord Changes in Mice. / Takano, Morito; Hikishima, Keigo; Fujiyoshi, Kanehiro; Shibata, Shinsuke; Yasuda, Akimasa; Konomi, Tsunehiko; Hayashi, Akiko; Baba, Hiroko; Honke, Koichi; Toyama, Yoshiaki; Okano, Hideyuki; Nakamura, Masaya.

In: PLoS One, Vol. 7, No. 12, e52904, 27.12.2012.

Research output: Contribution to journalArticle

Takano, M, Hikishima, K, Fujiyoshi, K, Shibata, S, Yasuda, A, Konomi, T, Hayashi, A, Baba, H, Honke, K, Toyama, Y, Okano, H & Nakamura, M 2012, 'MRI Characterization of Paranodal Junction Failure and Related Spinal Cord Changes in Mice', PLoS One, vol. 7, no. 12, e52904. https://doi.org/10.1371/journal.pone.0052904
Takano, Morito ; Hikishima, Keigo ; Fujiyoshi, Kanehiro ; Shibata, Shinsuke ; Yasuda, Akimasa ; Konomi, Tsunehiko ; Hayashi, Akiko ; Baba, Hiroko ; Honke, Koichi ; Toyama, Yoshiaki ; Okano, Hideyuki ; Nakamura, Masaya. / MRI Characterization of Paranodal Junction Failure and Related Spinal Cord Changes in Mice. In: PLoS One. 2012 ; Vol. 7, No. 12.
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