mRNA expression of the ATP-binding cassette transporter subfamily A (ABCA) in rat and human brain capillary endothelial cells

Sumio Ohtsuki, Yuki Watanabe, Satoko Hori, Hiroya Suzuki, Jiraganya Bhongsatiern, Masachika Fujiyoshi, Mayu Kamoi, Naoko Kamiya, Hitomi Takanaga, Tetsuya Terasaki

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The ATP-binding cassette transporter subfamily A (ABCA) consists of the transporters mediating cholesterol release and regulated by cholesterol. As about 25% of total body cholesterol exists in the brain, sterol homeostasis is an important issue as far as central nervous system function is concerned. The purpose of this study was to clarify the mRNA expression of ABCA subtypes at the blood-brain barrier (BBB) using cultured rat and human brain capillary endothelial cells, TR-BBB and hBME cells, respectively. mRNA expression of ABCA1, 2, 3, 4, 5, 6, 7 and 8/9 was detected in TR-BBB cells. In the brain capillary-rich fraction, mRNA expression of ABCA1, 2, 3, 4, 5, 7 and 8/9 was detected. ABCA2 and 5 mRNA were also detected in hBME cells. These results demonstrate, for the first time, that ABCA subtypes are expressed at the rat and/or human BBB. The expression of ABCA subtypes at the BBB is likely to contribute to sterol homeostasis in the central nervous system.

Original languageEnglish
Pages (from-to)1437-1440
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume27
Issue number9
DOIs
Publication statusPublished - 2004 Sep 1
Externally publishedYes

Keywords

  • ATP-binding cassette transporter subfamily A (ABCA)
  • Blood-brain barrier (BBB)
  • Brain capillary endothelial cells
  • TR-BBB
  • hBME
  • mRNA expression

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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    Ohtsuki, S., Watanabe, Y., Hori, S., Suzuki, H., Bhongsatiern, J., Fujiyoshi, M., Kamoi, M., Kamiya, N., Takanaga, H., & Terasaki, T. (2004). mRNA expression of the ATP-binding cassette transporter subfamily A (ABCA) in rat and human brain capillary endothelial cells. Biological and Pharmaceutical Bulletin, 27(9), 1437-1440. https://doi.org/10.1248/bpb.27.1437