mRNA sequence of the Xenopus laevis paxillin gene and its expression

Motoyuki Ogawa, Yoshiki Hiraoka, Koji Taniguchi, Yukinao Sakai, Sadakazu Aiso

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Paxillin is a cytoskeletal protein found in structures of focal adhesions where cells adhere to the extracellular matrix. We isolated paxillin cDNA from the Xenopus laevis ovary. The cDNA sequence encodes a protein of 539 amino acids with four LIM and five LD motifs. 80% of the amino acids of frog paxillin are shared by human and chicken paxillins. Northern analysis showed that the frog gene is expressed in the spleen, kidney, testis and ovary. Immunocytochemistry showed that paxillin protein is accumulated in the nucleus as well as in the periphery of the cytoplasm of the A6 cell. This intriguing result shows that paxillin, which has been characterized as a cytoskeletal protein, is capable of translocating to the nucleus.

Original languageEnglish
Pages (from-to)235-240
Number of pages6
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1519
Issue number3
DOIs
Publication statusPublished - 2001 Jun 28

Fingerprint

Paxillin
Xenopus laevis
Genes
Gene Expression
Messenger RNA
Cytoskeletal Proteins
Anura
Ovary
Complementary DNA
Amino Acids
Focal Adhesions
Cell adhesion
Extracellular Matrix
Testis
Chickens
Cytoplasm
Proteins
Spleen
Immunohistochemistry
Kidney

Keywords

  • LIM motif
  • Nuclear export signal
  • Nuclear translocation
  • Paxillin
  • Xenopus laevis

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

mRNA sequence of the Xenopus laevis paxillin gene and its expression. / Ogawa, Motoyuki; Hiraoka, Yoshiki; Taniguchi, Koji; Sakai, Yukinao; Aiso, Sadakazu.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1519, No. 3, 28.06.2001, p. 235-240.

Research output: Contribution to journalArticle

Ogawa, Motoyuki ; Hiraoka, Yoshiki ; Taniguchi, Koji ; Sakai, Yukinao ; Aiso, Sadakazu. / mRNA sequence of the Xenopus laevis paxillin gene and its expression. In: Biochimica et Biophysica Acta - Gene Structure and Expression. 2001 ; Vol. 1519, No. 3. pp. 235-240.
@article{e723ef7c0e084654910810c901405bdf,
title = "mRNA sequence of the Xenopus laevis paxillin gene and its expression",
abstract = "Paxillin is a cytoskeletal protein found in structures of focal adhesions where cells adhere to the extracellular matrix. We isolated paxillin cDNA from the Xenopus laevis ovary. The cDNA sequence encodes a protein of 539 amino acids with four LIM and five LD motifs. 80{\%} of the amino acids of frog paxillin are shared by human and chicken paxillins. Northern analysis showed that the frog gene is expressed in the spleen, kidney, testis and ovary. Immunocytochemistry showed that paxillin protein is accumulated in the nucleus as well as in the periphery of the cytoplasm of the A6 cell. This intriguing result shows that paxillin, which has been characterized as a cytoskeletal protein, is capable of translocating to the nucleus.",
keywords = "LIM motif, Nuclear export signal, Nuclear translocation, Paxillin, Xenopus laevis",
author = "Motoyuki Ogawa and Yoshiki Hiraoka and Koji Taniguchi and Yukinao Sakai and Sadakazu Aiso",
year = "2001",
month = "6",
day = "28",
doi = "10.1016/S0167-4781(01)00230-5",
language = "English",
volume = "1519",
pages = "235--240",
journal = "Biochimica et Biophysica Acta - Gene Structure and Expression",
issn = "0167-4781",
publisher = "Elsevier BV",
number = "3",

}

TY - JOUR

T1 - mRNA sequence of the Xenopus laevis paxillin gene and its expression

AU - Ogawa, Motoyuki

AU - Hiraoka, Yoshiki

AU - Taniguchi, Koji

AU - Sakai, Yukinao

AU - Aiso, Sadakazu

PY - 2001/6/28

Y1 - 2001/6/28

N2 - Paxillin is a cytoskeletal protein found in structures of focal adhesions where cells adhere to the extracellular matrix. We isolated paxillin cDNA from the Xenopus laevis ovary. The cDNA sequence encodes a protein of 539 amino acids with four LIM and five LD motifs. 80% of the amino acids of frog paxillin are shared by human and chicken paxillins. Northern analysis showed that the frog gene is expressed in the spleen, kidney, testis and ovary. Immunocytochemistry showed that paxillin protein is accumulated in the nucleus as well as in the periphery of the cytoplasm of the A6 cell. This intriguing result shows that paxillin, which has been characterized as a cytoskeletal protein, is capable of translocating to the nucleus.

AB - Paxillin is a cytoskeletal protein found in structures of focal adhesions where cells adhere to the extracellular matrix. We isolated paxillin cDNA from the Xenopus laevis ovary. The cDNA sequence encodes a protein of 539 amino acids with four LIM and five LD motifs. 80% of the amino acids of frog paxillin are shared by human and chicken paxillins. Northern analysis showed that the frog gene is expressed in the spleen, kidney, testis and ovary. Immunocytochemistry showed that paxillin protein is accumulated in the nucleus as well as in the periphery of the cytoplasm of the A6 cell. This intriguing result shows that paxillin, which has been characterized as a cytoskeletal protein, is capable of translocating to the nucleus.

KW - LIM motif

KW - Nuclear export signal

KW - Nuclear translocation

KW - Paxillin

KW - Xenopus laevis

UR - http://www.scopus.com/inward/record.url?scp=0035963486&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035963486&partnerID=8YFLogxK

U2 - 10.1016/S0167-4781(01)00230-5

DO - 10.1016/S0167-4781(01)00230-5

M3 - Article

C2 - 11418191

AN - SCOPUS:0035963486

VL - 1519

SP - 235

EP - 240

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

SN - 0167-4781

IS - 3

ER -