Mscs become collagen-type i producing cells with different phenotype in allogeneic and syngeneic bone marrow transplantation

Robert Maximilian Rusch, Yoko Ogawa, Shinri Sato, Satoru Morikawa, Emi Inagaki, Eisuke Shimizu, Kazuo Tsubota, Shigeto Shimmura

Research output: Contribution to journalArticlepeer-review

Abstract

Mesenchymal stem cells (MSCs) have been widely used in therapeutic applications for many decades. However, more and more evidence suggests that factors such as the site of origin and pre-implantation treatment have a crucial impact on the result. This study investigates the role of freshly isolated MSCs in the lacrimal gland after allogeneic transplantation. For this purpose, MSCs from transgenic GFP mice were isolated and transplanted into allogeneic and syngeneic recipients. While the syngeneic MSCs maintained a spherical shape, allogeneic MSCs engrafted into the tissue as spindle-shaped cells in the interstitial stroma. Furthermore, the MSCs produced collagen type I in more than 85% to 95% of the detected GFP+ MSCs in the recipients of both models, supposedly contributing to pathogenic fibrosis in allogeneic recipients compared to syngeneic models. These findings indicate that allogeneic MSCs act completely differently from syngeneic MSCs, highlighting the importance of understanding the exact mechanisms behind MSCs.

Original languageEnglish
Article number4895
JournalInternational journal of molecular sciences
Volume22
Issue number9
DOIs
Publication statusPublished - 2021 May 1

Keywords

  • Graft-versus-host disease (GVHD)
  • Lacrimal gland
  • Mesenchymal stem cells (MSCs)

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

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