MSI-1, a neural RNA-binding protein, is involved in male mating behaviour in Caenorhabditis elegans

Akinori Yoda, Hitoshi Sawa, Hideyuki Okano

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Background: Neural RNA-binding proteins are thought to play important roles in neural development and the functional regulation of postmitotic neurones by mediating post-transcriptional gene regulation. RNA-binding proteins belonging to the Musashi family are highly expressed in the nervous system; however, their roles are poorly understood. Results: We identified a Caenorhabditis elegans Musashi homologue, MSI-1, whose RNA-recognition motifs show extensive similarity to those of Drosophila and vertebrate Musashi proteins. We isolated a msi-1 mutant and found males with this mutation to have a mating defect. C. elegans male mating behaviour includes a distinct series of steps: response to contact, backing, turning, vulva location, spicule insertion, and sperm transfer. msi-1 is required for the turning and vulva location steps. Like other Musashi family members, MSI-1 is expressed specifically in neural cells, including male-specific neurones required for turning and vulva location. However, msi-1 was not expressed in proliferating neural progenitors in C. elegans, unlike the Musashi family genes in other systems. Conclusions: Our results suggest that msi-1 is expressed specifically in postmitotic neurones in C. elegans. msi-1 is required for full development of male mating behaviour, possibly through regulation of msi-1 expressing neurones.

Original languageEnglish
Pages (from-to)885-895
Number of pages11
JournalGenes to Cells
Volume5
Issue number11
DOIs
Publication statusPublished - 2000
Externally publishedYes

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RNA-Binding Proteins
Caenorhabditis elegans
Vulva
Neurons
Nervous System
Genes
Vertebrates
Spermatozoa
Mutation
N-methylsuccinimide

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

MSI-1, a neural RNA-binding protein, is involved in male mating behaviour in Caenorhabditis elegans. / Yoda, Akinori; Sawa, Hitoshi; Okano, Hideyuki.

In: Genes to Cells, Vol. 5, No. 11, 2000, p. 885-895.

Research output: Contribution to journalArticle

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