MTOR complex 2 controls glycolytic metabolism in glioblastoma through FoxO acetylation and upregulation of c-Myc

Kenta Masui, Kazuhiro Tanaka, David Akhavan, Ivan Babic, Beatrice Gini, Tomoo Matsutani, Akio Iwanami, Feng Liu, Genaro R. Villa, Yuchao Gu, Carl Campos, Shaojun Zhu, Huijun Yang, William H. Yong, Timothy F. Cloughesy, Ingo K. Mellinghoff, Webster K. Cavenee, Reuben J. Shaw, Paul S. Mischel

Research output: Contribution to journalArticle

178 Citations (Scopus)

Abstract

Aerobic glycolysis (the Warburg effect) is a core hallmark of cancer, but the molecular mechanisms underlying it remain unclear. Here, we identify an unexpected central role for mTORC2 in cancer metabolic reprogramming where it controls glycolytic metabolism by ultimately regulating the cellular level of c-Myc. We show that mTORC2 promotes inactivating phosphorylation of class IIa histone deacetylases, which leads to the acetylation of FoxO1 and FoxO3, and this in turn releases c-Myc from a suppressive miR-34c-dependent network. These central features of activated mTORC2 signaling, acetylated FoxO, and c-Myc levels are highly intercorrelated in clinical samples and with shorter survival of GBM patients. These results identify a specific, Akt-independent role for mTORC2 in regulating glycolytic metabolism in cancer.

Original languageEnglish
Pages (from-to)726-739
Number of pages14
JournalCell Metabolism
Volume18
Issue number5
DOIs
Publication statusPublished - 2013 Nov 5
Externally publishedYes

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Glioblastoma
Acetylation
Up-Regulation
Neoplasms
Histone Deacetylases
Glycolysis
Phosphorylation
Survival
TOR complex 2

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Physiology

Cite this

Masui, K., Tanaka, K., Akhavan, D., Babic, I., Gini, B., Matsutani, T., ... Mischel, P. S. (2013). MTOR complex 2 controls glycolytic metabolism in glioblastoma through FoxO acetylation and upregulation of c-Myc. Cell Metabolism, 18(5), 726-739. https://doi.org/10.1016/j.cmet.2013.09.013

MTOR complex 2 controls glycolytic metabolism in glioblastoma through FoxO acetylation and upregulation of c-Myc. / Masui, Kenta; Tanaka, Kazuhiro; Akhavan, David; Babic, Ivan; Gini, Beatrice; Matsutani, Tomoo; Iwanami, Akio; Liu, Feng; Villa, Genaro R.; Gu, Yuchao; Campos, Carl; Zhu, Shaojun; Yang, Huijun; Yong, William H.; Cloughesy, Timothy F.; Mellinghoff, Ingo K.; Cavenee, Webster K.; Shaw, Reuben J.; Mischel, Paul S.

In: Cell Metabolism, Vol. 18, No. 5, 05.11.2013, p. 726-739.

Research output: Contribution to journalArticle

Masui, K, Tanaka, K, Akhavan, D, Babic, I, Gini, B, Matsutani, T, Iwanami, A, Liu, F, Villa, GR, Gu, Y, Campos, C, Zhu, S, Yang, H, Yong, WH, Cloughesy, TF, Mellinghoff, IK, Cavenee, WK, Shaw, RJ & Mischel, PS 2013, 'MTOR complex 2 controls glycolytic metabolism in glioblastoma through FoxO acetylation and upregulation of c-Myc', Cell Metabolism, vol. 18, no. 5, pp. 726-739. https://doi.org/10.1016/j.cmet.2013.09.013
Masui, Kenta ; Tanaka, Kazuhiro ; Akhavan, David ; Babic, Ivan ; Gini, Beatrice ; Matsutani, Tomoo ; Iwanami, Akio ; Liu, Feng ; Villa, Genaro R. ; Gu, Yuchao ; Campos, Carl ; Zhu, Shaojun ; Yang, Huijun ; Yong, William H. ; Cloughesy, Timothy F. ; Mellinghoff, Ingo K. ; Cavenee, Webster K. ; Shaw, Reuben J. ; Mischel, Paul S. / MTOR complex 2 controls glycolytic metabolism in glioblastoma through FoxO acetylation and upregulation of c-Myc. In: Cell Metabolism. 2013 ; Vol. 18, No. 5. pp. 726-739.
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