Mucosal expression of aquaporin-4 in the stomach of histamine type 2 receptor knockout mice and Helicobacter pylori-infected mice

Seiichiro Fukuhara, Juntaro Matsuzaki, Hitoshi Tsugawa, Tatsuhiro Masaoka, Sawako Miyoshi, Hideki Mori, Yasushi Fukushima, Masato Yasui, Takanori Kanai, Hidekazu Suzuki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Background and Aim: Basolateral water channel, aquaporin-4 (AQP4), is known to be expressed in gastric parietal cells, especially in the basal side of gastric mucosa. However, the role of AQP4 in the stomach is still unknown. Histamine type 2 receptor (H2R) knockout mice, which are characterized by suppressed gastric acid secretion, are known as formation of mucosal hyperplasia with cystic dilatation and spasmolytic polypeptide-expressing metaplasia (SPEM) in the stomach. The aim of the present study is to investigate whether the expression of AQP4 is changed by the condition of acid suppression and Helicobacter pylori infection. Methods: Male H2R knockout mice and their controls (C57BL/6) were used. H.pylori was orally infected at the age of 5 weeks. The distributions of AQP4 and H+/K+-ATPase in the gastric mucosa were investigated by fluorescent immunohistochemistry. The mRNA expressions of AQP4, H+/K+- ATPase, sonic hedgehog (Shh), and trefoil factor-2 (TFF2) were investigated by quantitative reverse transcription polymerase chain reaction (RT-PCR). Results: In the H2R knockout mice, the distribution of AQP4-positive parietal cells was extended toward the surface of the fundic glands. Although the mRNA expression levels of AQP4 and H+/K+ ATPase were elevated in H2R knockout mice at the age of 20 weeks, the elevations were not maintained by aging or H.pylori infection. In H2R knockout mice with H.pylori infection, the expression level of TFF2mRNA was elevated while the ratio between AQP4 and H+/K+ ATPase mRNA expression was decreased compared with the H2R knockout mice without H.pylori infection. Conclusions: In the H2R knockout mice, massive SPEM was induced by H.pylori colonization and the ratio between AQP4 and H+/K+ ATPase mRNA expression was decreased.

Original languageEnglish
Pages (from-to)53-59
Number of pages7
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume29
Issue numberS4
DOIs
Publication statusPublished - 2014 Dec 1

Fingerprint

Aquaporin 4
Histamine Receptors
Knockout Mice
Helicobacter pylori
Stomach
Proton-Translocating ATPases
Pylorus
Messenger RNA
Metaplasia
Gastric Mucosa
Infection
Gastric Parietal Cells
Aquaporins
Hedgehogs
Gastric Acid
Helicobacter Infections
Reverse Transcription
Hyperplasia
Dilatation
Immunohistochemistry

Keywords

  • Acid suppression
  • Aquaporin-4
  • Helicobacter pylori
  • SPEM

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

Cite this

Mucosal expression of aquaporin-4 in the stomach of histamine type 2 receptor knockout mice and Helicobacter pylori-infected mice. / Fukuhara, Seiichiro; Matsuzaki, Juntaro; Tsugawa, Hitoshi; Masaoka, Tatsuhiro; Miyoshi, Sawako; Mori, Hideki; Fukushima, Yasushi; Yasui, Masato; Kanai, Takanori; Suzuki, Hidekazu.

In: Journal of Gastroenterology and Hepatology (Australia), Vol. 29, No. S4, 01.12.2014, p. 53-59.

Research output: Contribution to journalArticle

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T1 - Mucosal expression of aquaporin-4 in the stomach of histamine type 2 receptor knockout mice and Helicobacter pylori-infected mice

AU - Fukuhara, Seiichiro

AU - Matsuzaki, Juntaro

AU - Tsugawa, Hitoshi

AU - Masaoka, Tatsuhiro

AU - Miyoshi, Sawako

AU - Mori, Hideki

AU - Fukushima, Yasushi

AU - Yasui, Masato

AU - Kanai, Takanori

AU - Suzuki, Hidekazu

PY - 2014/12/1

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N2 - Background and Aim: Basolateral water channel, aquaporin-4 (AQP4), is known to be expressed in gastric parietal cells, especially in the basal side of gastric mucosa. However, the role of AQP4 in the stomach is still unknown. Histamine type 2 receptor (H2R) knockout mice, which are characterized by suppressed gastric acid secretion, are known as formation of mucosal hyperplasia with cystic dilatation and spasmolytic polypeptide-expressing metaplasia (SPEM) in the stomach. The aim of the present study is to investigate whether the expression of AQP4 is changed by the condition of acid suppression and Helicobacter pylori infection. Methods: Male H2R knockout mice and their controls (C57BL/6) were used. H.pylori was orally infected at the age of 5 weeks. The distributions of AQP4 and H+/K+-ATPase in the gastric mucosa were investigated by fluorescent immunohistochemistry. The mRNA expressions of AQP4, H+/K+- ATPase, sonic hedgehog (Shh), and trefoil factor-2 (TFF2) were investigated by quantitative reverse transcription polymerase chain reaction (RT-PCR). Results: In the H2R knockout mice, the distribution of AQP4-positive parietal cells was extended toward the surface of the fundic glands. Although the mRNA expression levels of AQP4 and H+/K+ ATPase were elevated in H2R knockout mice at the age of 20 weeks, the elevations were not maintained by aging or H.pylori infection. In H2R knockout mice with H.pylori infection, the expression level of TFF2mRNA was elevated while the ratio between AQP4 and H+/K+ ATPase mRNA expression was decreased compared with the H2R knockout mice without H.pylori infection. Conclusions: In the H2R knockout mice, massive SPEM was induced by H.pylori colonization and the ratio between AQP4 and H+/K+ ATPase mRNA expression was decreased.

AB - Background and Aim: Basolateral water channel, aquaporin-4 (AQP4), is known to be expressed in gastric parietal cells, especially in the basal side of gastric mucosa. However, the role of AQP4 in the stomach is still unknown. Histamine type 2 receptor (H2R) knockout mice, which are characterized by suppressed gastric acid secretion, are known as formation of mucosal hyperplasia with cystic dilatation and spasmolytic polypeptide-expressing metaplasia (SPEM) in the stomach. The aim of the present study is to investigate whether the expression of AQP4 is changed by the condition of acid suppression and Helicobacter pylori infection. Methods: Male H2R knockout mice and their controls (C57BL/6) were used. H.pylori was orally infected at the age of 5 weeks. The distributions of AQP4 and H+/K+-ATPase in the gastric mucosa were investigated by fluorescent immunohistochemistry. The mRNA expressions of AQP4, H+/K+- ATPase, sonic hedgehog (Shh), and trefoil factor-2 (TFF2) were investigated by quantitative reverse transcription polymerase chain reaction (RT-PCR). Results: In the H2R knockout mice, the distribution of AQP4-positive parietal cells was extended toward the surface of the fundic glands. Although the mRNA expression levels of AQP4 and H+/K+ ATPase were elevated in H2R knockout mice at the age of 20 weeks, the elevations were not maintained by aging or H.pylori infection. In H2R knockout mice with H.pylori infection, the expression level of TFF2mRNA was elevated while the ratio between AQP4 and H+/K+ ATPase mRNA expression was decreased compared with the H2R knockout mice without H.pylori infection. Conclusions: In the H2R knockout mice, massive SPEM was induced by H.pylori colonization and the ratio between AQP4 and H+/K+ ATPase mRNA expression was decreased.

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KW - Aquaporin-4

KW - Helicobacter pylori

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