Mullerian Inhibiting Substance is present in embryonic testes of dogs with Persistent Mullerian Duct Syndrome

V. N. Meyers-Wallen, M. M. Lee, T. F. Manganaro, Tatsuo Kuroda, D. MacLaughlin, P. K. Donahoe

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Mullerian Inhibiting Substance (MIS) causes regression of the Mullerian ducts during a critical period in embryonic development in male mammals. In Persistent Mullerian Duct Syndrome (PMDS), an autosomal recessive trait in humans and dogs, the Mullerian ducts fail to regress in otherwise normal males. Previously we reported that PMDS-affected dogs produce bioactive testicular MIS postnatally. The purpose of the present study was to determine whether PMDS-affected canine embryos appropriately express MIS mRNA and protein during the critical period for Mullerian duct regression. Homozygous (PMDS-affected) and normal canine embryos were removed from timed pregnancies. Gonadal sex and the degree of Mullerian duct regression were determined from histologic sections. Positive immunohistochemical staining for MIS was found in testis sections of PMDS-affected and normal male embryos. A 1.8-kb MIS mRNA transcript was detected in testes of PMDS-affected males and normal male embryos and neonates. Furthermore, equal amounts of MIS mRNA transcript were detected in testes of PMDS-affected embryos and normal male littermates during the critical period for Mullerian duct regression. These data support a hypothesis of target organ resistance, such as an abnormality in the putative MIS receptor, as the etiology of the defect in this dog model.

Original languageEnglish
Pages (from-to)1410-1418
Number of pages9
JournalBiology of Reproduction
Volume48
Issue number6
DOIs
Publication statusPublished - 1993
Externally publishedYes

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Anti-Mullerian Hormone
Mullerian Ducts
Testis
Dogs
Embryonic Structures
Messenger RNA
Canidae
Persistent Müllerian Duct Syndrome
Embryonic Development
Mammals
Staining and Labeling
Pregnancy

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

Cite this

Mullerian Inhibiting Substance is present in embryonic testes of dogs with Persistent Mullerian Duct Syndrome. / Meyers-Wallen, V. N.; Lee, M. M.; Manganaro, T. F.; Kuroda, Tatsuo; MacLaughlin, D.; Donahoe, P. K.

In: Biology of Reproduction, Vol. 48, No. 6, 1993, p. 1410-1418.

Research output: Contribution to journalArticle

Meyers-Wallen, V. N. ; Lee, M. M. ; Manganaro, T. F. ; Kuroda, Tatsuo ; MacLaughlin, D. ; Donahoe, P. K. / Mullerian Inhibiting Substance is present in embryonic testes of dogs with Persistent Mullerian Duct Syndrome. In: Biology of Reproduction. 1993 ; Vol. 48, No. 6. pp. 1410-1418.
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