Mult-component analysis of urine of dogs with liver diseases by high-performance liquid chromatography

Yoshiko Abe, Eriko Kobayashi, Noriko Suzuki, Akiko Kaneko, Tadao Fujie, Yoshiko Nagata, Yoshikazu Matsushima, Nobuharu Takai, Toshifumi Watanabe, Osamu Otsubo

Research output: Contribution to journalArticlepeer-review

Abstract

Components of the urine of normal dogs and dogs with liver diseases were analysed by high-performance liquid chromatography (HPLC) using a macroreticular anion-exchange resin with UV detection. The dogs with liver diseases were experimentally prepared by either Eck fistula by portal-caval anastomosis, dimethylnitrosamine-induced liver failure or total hepatectomy by operation. Eighteen chromatographic peaks were numbered and the cross-correlation coefficient of the intensities of every pair of peaks was computed. There were high correlations between several pairs of peaks according to the types and degrees of the hepatic failures, whereas no significant correlation was observed for the peak pairs of normal urine samples. The results showed that HPLC analysis of urine is useful in the diagnosis of the physiological state in liver dysfunction.

Original languageEnglish
Pages (from-to)11-17
Number of pages7
JournalJournal of Chromatography A
Volume386
Issue numberC
DOIs
Publication statusPublished - 1987
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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