Multidrug resistance in Klebsiella pneumoniae MGH78578 and cloning of genes responsible for the resistance

Wakano Ogawa, Dai Wei Li, Ping Yu, Anowara Begum, Tohru Mizushima, Teruo Kuroda, Tomofusa Tsuchiya

Research output: Contribution to journalArticlepeer-review

37 Citations (Scopus)

Abstract

Klebsiella pneumoniae MGH78578, a clinical isolate, showed high level of resistance to many antimicrobial agents. We cloned genes responsible for drug resistance from chromosomal DNA of K. pneumoniae MGH78578 by shotgun method using Escherichia coli KAM32, a drug hypersensitive strain, as host. We obtained 43 hybrid plasmids that made host cells resistant to several antimicrobial agents. We classified them into 17 groups based on growth properties in the presence of each one of 9 antimicrobial agents and on restriction patterns of each hybrid plasmid. Analysis of the cloned genes must be very useful for investigation of major parts of multidrug resistance systems including multidrug efflux pumps in K. pneumoniae MGH78578 in which genome sequence is available.

Original languageEnglish
Pages (from-to)1505-1508
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume28
Issue number8
DOIs
Publication statusPublished - 2005 Aug

Keywords

  • Gene cloning
  • Klebsiella pneumoniae
  • Multidrug efflux pump
  • Multidrug resistance

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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