Multidrug resistance in Serratia marcescens and cloning of genes responsible for the resistance.

Jing Chen, Eun Woo Lee, Teruo Kuroda, Tohru Mizushima, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Six clinically isolated strains of Serratia marcescens were tested for their drug resistance. All showed fairly high resistance to many antimicrobial agents tested including norfloxacin, streptomycin, ampicillin, erythromycin, tetracycline, chloramphenicol, and antimicrobial dyes. Using the drug-hypersensitive strain of Escherichia coli KAM32 as the host, we cloned the genes responsible for multidrug resistance from chromosomal DNA of one of the strains of S. marcescens, NUSM8906. We obtained 28 hybrid plasmids that made host cells resistant to several antimicrobial agents. Many of the transformants harboring each of the plasmids showed multidrug resistance, and some showed resistance to specific drugs. The hybrid plasmids were classified into several groups based on their drug specificity. It appears that each class of plasmid carries different types of drug resistance genes. Analysis of such genes will reveal the multiple mechanisms involved in multidrug resistance in S. marcescens.

Original languageEnglish
Pages (from-to)391-393
Number of pages3
JournalBiological & pharmaceutical bulletin
Volume26
Issue number3
Publication statusPublished - 2003 Mar
Externally publishedYes

Fingerprint

Serratia marcescens
Multiple Drug Resistance
Organism Cloning
Plasmids
Anti-Infective Agents
Drug Resistance
Genes
Pharmaceutical Preparations
MDR Genes
Norfloxacin
Streptomycin
Chloramphenicol
Erythromycin
Ampicillin
Tetracycline
Coloring Agents
Escherichia coli
DNA

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Chen, J., Lee, E. W., Kuroda, T., Mizushima, T., & Tsuchiya, T. (2003). Multidrug resistance in Serratia marcescens and cloning of genes responsible for the resistance. Biological & pharmaceutical bulletin, 26(3), 391-393.

Multidrug resistance in Serratia marcescens and cloning of genes responsible for the resistance. / Chen, Jing; Lee, Eun Woo; Kuroda, Teruo; Mizushima, Tohru; Tsuchiya, Tomofusa.

In: Biological & pharmaceutical bulletin, Vol. 26, No. 3, 03.2003, p. 391-393.

Research output: Contribution to journalArticle

Chen, J, Lee, EW, Kuroda, T, Mizushima, T & Tsuchiya, T 2003, 'Multidrug resistance in Serratia marcescens and cloning of genes responsible for the resistance.', Biological & pharmaceutical bulletin, vol. 26, no. 3, pp. 391-393.
Chen, Jing ; Lee, Eun Woo ; Kuroda, Teruo ; Mizushima, Tohru ; Tsuchiya, Tomofusa. / Multidrug resistance in Serratia marcescens and cloning of genes responsible for the resistance. In: Biological & pharmaceutical bulletin. 2003 ; Vol. 26, No. 3. pp. 391-393.
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